Download Gene for Gene theory

Koch’ Postulates
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병원균과 질병의 지속적 연관성
분리, 순수배양
건강한 개체에 접종시 동일한 증상
재분리 후 동일한 병원균 확인
Plant vs. Animal
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No antibody, T cell response
No circulating cell
Systemic signal through plant
Disposable and replaceable parts in
plant
Gene for Gene theory
• Avr : avr (avirulence) gene
• R : r (resistance) gene
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R * Avr = Resistance
R * avr = Susceptible
r * Avr = Susceptible
R * avr = Susceptible
Signal Molecules
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ROD, reactive oxygen species
NO, nitric oxide
SA, salicylic acid
JA, jasmoic acid
C2H2, ethylene
신호전달 후
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HR, hypersensive response
Phytoalexins
PR, pathogenesis related proteins
Cell wall reinforcing, H2O2
Ca++
Active oxygens
SA
R and Avr interactions
• PAMP(pathogen associated molecular pattern)
through TTSS(type III secretion system), Fls2(flagellin
receptor)
• AvrRpm1 increases phosphorylation of Rin4 and
decrease PAMP signaling
• AvrRpt2, protease cleaves Rin4
• Rpm1 interact with Rin4 and detect change in Rin4
내병성 육종
Exogeneous genes
- pathogen origin (M, Coat proteins,
replicase)
- plant origin (RIP)
Endogenous genes
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SAR
HR
PR
Signal transduction
내병성 분자육종
• Engineering of R for broad spectrum
• Expression of R in an inducible manner
- broad specificity of pathogens
- multifactorial defense mechanism
- only w/I infected tissue
• 내병성 육종의 한계: 병원균의 진화