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Cells Wassily Kandinsky (1866-1944) Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cell Theory: - all organisms are composed of cells - all cells come from other cells Figure 4.1x Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • Scanning electron microscope (SEM) TEM Figure 4.1B Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • Cell size and shape relate to function • Minimum • Maximum Figure 4.2 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • A small cell has a greater ratio of surface area to volume than a large cell of the same shape 30 µm Figure 4.3 Surface area of one large cube = 5,400 µm2 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings 10 µm Total surface area of 27 small cubes = 16,200 µm2 2 kinds of cells: prokaryotic and eukaryotic • Prokaryotic cells - “before nucleus” - small, relatively simple cells – Single-celled organisms – May not require oxygen – No organelles (with membranes) Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • A prokaryotic cell has: - plasma membrane - rigid cell wall – sticky capsule – Nucleoid region with DNA Capsule Cell wall Plasma membrane – Some w/ flagella Pili Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Prokaryotic flagella Ribosomes Nucleoid region (DNA) Figure 4.4 • Prokaryotic cells, Bacillus polymyxa Figure 4.4x1 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Eukaryotic cells - functional compartments - true nucleus - larger: 10-100 microns - often multicellular - organelles surrounded by membranes - usually need O2 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Endomembrane System • a group of organelles that manufactures and distributes cell products • nucleus • endoplasmic reticulum (ER), ribosomes • Golgi complex, vesicles Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings ENDOMEMBRANE SYSTEM nucleus is the control center • largest organelle • nuclear envelope • contains DNA that directs cell’s activities • DNA copy goes into every progeny cell Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings NUCLEUS Chromatin Nucleolus Two membranes of nuclear envelope Pore ROUGH ENDOPLASMIC RETICULUM Ribosomes Figure 4.6 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings nucleus nuclear pores Rough endoplasmic reticulum • Makes proteins, membranes Transport vesicle buds off 4 osome Sugar chain 1 Polypeptide 3 Secretory (glyco-) protein inside transport vesicle Glycoprotein 2 ROUGH ER Figure 4.8 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings SMOOTH ER ROUGH ER Nuclear envelope Ribosomes SMOOTH ER Figure 4.9 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings ROUGH ER • The Golgi complex finishes, sorts, and ships cell products Golgi apparatus Golgi apparatus “Receiving” side of Golgi apparatus Transport vesicle from ER New vesicle forming “Shipping” side of Golgi apparatus Transport vesicle from the Golgi Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Figure 4.10 Lysosomes • sacs of digestive enzymes budded off the Golgi • Fuse with membrane around debris LYSOSOME Nucleus Figure 4.11A Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • Lysosomal enzymes – digest food – destroy bacteria – recycle damaged organelles – function in embryonic development in animals – Storage of undigestable waste Pombe’s disease - glycogen Tay-Sachs disease - lipids Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Rough ER Transport vesicle (containing inactive hydrolytic enzymes) Plasma membrane Golgi apparatus Engulfment of particle Lysosome engulfing damaged organelle “Food” LYSOSOMES Food vacuole Figure 4.11B Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Digestion Smooth endoplasmic reticulum • synthesizes lipids • regulates carbohydrate metabolism (liver) • breaks down toxins and drugs (liver) • Stores Ca++ in muscle cells Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Endomembrane system • Nucleus, ribosomes, RER ,SER, Golgi, vesicles Rough ER Transport vesicle from Golgi Transport vesicle from ER Plasma membrane Vacuole Nucleus Lysosome Smooth ER Nuclear envelope Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Golgi apparatus Figure 4.14 • Vacuole in plants – Lysosomal – storage of pigments, poisons Central vacuole – Water relations Nucleus Figure 4.13A Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • plant cells – large central vacuole – rigid cell wall – chloroplasts Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Chloroplasts • in plants and some protists • convert solar energy to chemical energy in sugars Chloroplast Stroma Inner and outer membranes Granum Figure 4.15 Intermembrane space Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • cellular respiration Mitochondrion Outer membrane Intermembrane space Inner membrane Cristae Figure 4.16 Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Matrix The CYTOSKELETON helps organize a cell’s structure and activities • network of protein fibers Figure 4.17A Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings microfilament Intermediate filament microtubule 25 nm 10 nm 7 nm Actin subunit Cell shape, movement Fibrous subunits reinforce cell, anchor organelles Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Tubulin subunit cell rigidity, anchor & tracks for organelles, mitosis • Nuclei (yellow) and actin (red) Figure 4.6x Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cilia and flagella • locomotor appendages • composed of a core of microtubules wrapped in the plasma membrane Basal body Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cell surfaces protect, support, and join cells Surfaces allow exchange of signals and molecules. • Plant cells connect by plasmodesmata Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Walls of two adjacent plant cells Vacuole PLASMODESMATA Layers of one plant cell wall Cytoplasm Plasma membrane Figure 4.19A Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • Animal cells - surrounded by an extracellular matrix –sticky layer of glycoproteins –binds cells together in tissues –can also protect and support cells Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings • Tight junctions can bind cells together into leakproof sheets • Anchoring junctions link animal cells TIGHT JUNCTION ANCHORING JUNCTION • Gap junctions allow substances to flow from cell to cell COMMUNICATING JUNCTION Plasma membranes of adjacent cells Figure 4.19B Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Extracellular matrix Eukaryotic organelles fall into 4 functional groups • 1. Manufacture and transport – dependent on network of membranes - Nucleus - Ribosomes - Rough, smooth ER - Golgi apparatus Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings 2. Breakdown – all single-membrane sacs • Lysosomes (in animals, some protists) • Peroxisomes • Vacuoles (plants) Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings 3. Energy Processing – involves extensive membranes embedded with enzymes • Chloroplasts • Mitochondria Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings 4. Support, Movement, Communication • Cytoskeleton – includes cilia, flagella, filaments, microtubules • Cell walls • Extracellular matrix • Cell junctions Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings