Download Tianyuan He, MD, PhD 6447 Med Sci I 0609 734-528-2407(H), 734-635-0269(Cell)

Tianyuan He, MD, PhD
6447 Med Sci I 0609
1301 E. Catherine St. Ann Arbor, MI 48109
734-528-2407(H), 734-635-0269(Cell)
[email protected]
EDUCATION
9/2001-5/2002
9/1994-7/1997
3/1991-6/1994
10/1978-7/1983
Mo’s Computer Science Institute / National Bioinformatics Institute., CA, USA,
Bioinformatics Specialist / Master
Shanghai Second Medical University (SSMU), Shanghai, P.R. China, PhD in
Molecular Biology
Nanjing Medical University, Nanjing, Jiansu, P.R. China, Master degree in
Biochemistry
Bengbu Medical College (BBMC), Bengbu, Anhui, P.R. China, MD in Medicine
POSTDOCTORAL TRAINING
4/1998-4/2003 Department of Dermatology, University of Michigan
ACADEMIC APPOINTMENTS
9/2013-present
5/2008-8/2013
5/2003-5/2008
7/1997-4/1998
6/1992-8/1994
2/1990-3/1991
7/1987-2/1990
Assistant Research Scientist, Department of Dermatology, University of Michigan
Research Investigator, Department of Dermatology, University of Michigan
Research Lab Specialist Senior, Department of Dermatology, University of
Michigan
Researcher and Lecturer, Research Center for Human Gene Therapy, SSMU, P. R.
China
Vice chairman, Department of Biochemistry, Bengbu Medical College (BBMC),
Bengbu, Anhui, P. R. China
Researcher and Lecturer, Department of Biochemistry, Bengbu Medical College
(BBMC), Bengbu, Anhui, P. R. China
Researcher and Teaching Assistant, Department of Biochemistry, Bengbu Medical
College (BBMC), Bengbu, Anhui, P. R. China
Technical Skills
Routine manipulation
Isolation and purification of protein, genomic DNA and RNA. Gene cloning and sequencing; Southern,
Northern and Western blot, ELISA; in situ hybridization; Immunoprecipitation (IP and co-IP); gene
cloning, sub-cloning and mutagenesis; generation of vectors for various expression systems (bacterial and
mammalian); evaluation of gene expression and interactions by CHIP assay, nuclear run-on/off
transcription assay, siRNA, shRNA and MicroRNA design and experimental performance; functional
analysis of gene promoter by serial deletion analysis, electrophoretic mobility shift assay, footprint,
DAPA; generation of transient and stable cell lines using multiple transfection techniques, such as
retrovirus, adenovirus, lentivirus, lipid based reagents, or electroporation on cell lines and primary
cultured cells; flow cytometry, immunoprecipitation, and fluorescence confocal microscopy, etc
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High throughput manipulation
Establishing gene expression profiles on genome wide scale from small experiment samples (like LCM)
by RNA extraction and amplification system coupled with microarray and / or real time quantitative RTPCR;
Other manipulation
Ability to manipulate any existing or novel experiments
Knowledge of computer system and bioinformatics:
Extensive knowledge of microsofe Word, Excel, Access, PowerPoint, FrontPage,
Good knowledge of microarray data analysis.
SCIENTIFIC ACTIVITIES
2004-2012
Invited paper reviewer for Journal of Investigative Dermatology
Invited paper reviewer for Cancer Biology & Therapy
Invited Fund reviewer for Genome British Columbia in Canada
2012-2013
GRANT SUPPORT
Curent:
National Institute of Health, RO1
Key Person
“TGF-ß Signaling in Photoaged and Chronologically Aged Human Skin”
02/01/02 – 04/30/2015
National Institute of Health, RO1
Key Person
“Regulation of Extracellular Matrix Homeostasis in Skin Aging”
08/15/2009 - 07/31/2014
Past:
National Institute of Health, RO1
Key Person
“Transforming Growth Factor-Beta (TGF-B) Signaling in Photoaged and Chronologically
Aged Human Skin”
01/18/2002 – 01/31-2008
Johnson & Johnson
Key Person
"Molecular Mechanisms of Skin Aging and Repair "
01/01/91 - 1/31/03
National Natural Science Foundation of China (Research Grant)
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Principal Investigator
“Direct transfer of the VEGF gene into myocardium for the gene therapy of ischemic
heart disease”
1995
Education Commission of Anhui Province, China (Research Grant)
Principal Investigator
“The expression of mutant oncoprotein p53 in tumor tissue”
1993
Education Commission of Anhui Province, China (Research Grant)
Principal Investigator
“ Serial researches of asparagus on repression of tumor growth”
1992
CERTIFICATION AND LICENSURE
NIH Office of Human Subjects Research Certificate
MEMBERSHIPS AND OFFICES IN PROFESSIONAL SOCIETIES
1985-present Chinese Society of Molecular Biology and Biochemistry
1999-present American Association for the Advancement of Science
2000-present Society for Investigative Dermatology
TEACHING ACTIVITIES
1999
2002
2002
Mentor of Lars Estrom (undergraduate student)
Undergraduate Research Opportunity Program – Mentor of Nicole Prince
U of Toronto Summer Scholarship Program – Mentor of Sharon Shin
EXTRAMURAL INVITED PRESENTATIONS
“Acute oxidative stress causes long-lived reduction of type I collagen and increased collagenase in human skin
fibroblasts”, Society for Investigative Dermatology, 66th Annual Meeting, America’s Center, St. Louis,
Missouri. May 4-7, 2005.
“UV irradiation inhibits procollagen synthesis by down-regulating the TGF-ß type II receptor in human skin
fibroblasts”, Bengbu Medical College, Anhui, PR China, March 16, 2005.
“UV irradiation inhibits procollagen synthesis by down-regulating the TGF-ß type II receptor in human skin
fibroblasts”, Shanghai Second Medical University, Shanghai PR China, March 24, 2005.
“Oxidative stress impairs TGF- responsiveness by coordinate down-regulation of Type II TGF- receptor and
smad3 in primary human skin fibroblasts”, (Plenary session) Society for Investigative Dermatology, May 6-9,
2009 Montreal, Canada.
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BIBLIOGRAPHY
Completed Publications in Scientific Journals
Peer Reviewed Publications
1. Zhiyi Chen, Kai Xue, Tianyuan He, Xuehai Zhao. Methodological Studies of Determination of Serum
Antitrypsin Inhibitory Capacity Acta Academiae Medicine, Bengbu, 13(4): 159 (1988)
2. Anchao Wang, Tianyuan He. Diagnostic Value of Determining Serum Lipid-bound Sialic Acid in
Pulmonary Diseases Acta Academiae Medicine, Bengbu, 15(4): 239 (1990)
3. Xue Kai, Tianyuan He, Yiying Wu, Zhiyi Chen,. Preliminary Application of Serum Lipidbound Sialic
Acid in Clinical Diagnosis of Malignancy Acta Academiae Medicine, Bengbu 15(2): 75 (1990)
4. Tianyuan He, Fen Xu,. Advances in the Research of Tyrosine Protein Phosphatase (Rewiew Article)
Foreign Medical Sciences in Molecular Biology 15(2):84 (1993)
5. Tianyuan He, Shishu Chen,. Advances in VEGF and Angiogenesis (Review Article) Foreign Medical
Sciences in Molecular Biology 19(5): 236-239 (1997)
6. Tianyuan He, Hushen Qian, Shishu Chen,. A Study on the Expression of VEGF cDNA Mediated by
lipofectAMINE in CHO cells Acta Universitatis Medicinalis Secondae Shanghai 18(2): 92-95 (1998)
7. Zhao Y, Shi Y, He T, Zhang H, Chen S, Zhang B,. Direct Intramuscular Injection of pSV-VEGF(165)
Augments Capillaries Formation in a Rabbit Model of Hindlimb Ischemia. Zhonghua Wai Ke Za Zhi
37(4):211 (1999)
8. Tianyuan He, Yaxue Shi, Shishu Chen,. Expression of VEGF on Rabbit Skin Fibroblasts Using
Retroviral Vector. Acta Biological Experimentalis Sinica, 33(2): 101-107 (2000)
9. Taihao Quan*, Tianyuan He*, John Voorhees, Gary J. Fisher,. Ultraviolet Irradiation Blocks Cellular
Responses to Transforming Growth Factor- by Down-regulating Its Type-II Receptor and Inducing
Smad7 Journal of Biological Chemistry 276; 26349-26356 (2001)
10. Taihao Quan*, Tianyuan He*, Sewon Kang, John J. Voorhees, Gary J. Fisher,. Connective Tissue
Growth Factor: Expression in Human Skin in vivo and Inhibition by Ultraviolet Irradiation. Journal of
Investigative Dermatology, 118; 402-408, (2002)
11. Taihao Quan*, Tianyuan He*, Sewon Kang, John J. Voorhees, Gary J. Fisher,. Ultraviolet
Irradiation Alters Transforming Growth Factor-/Smad Pathway in Human Skin in vivo. Journal of
Investigative Dermatology 119; 499-506, (2002)
12. Hui Qin Wang, Taihao Quan, Tianyuan He, Thomas F. Franke, John J. Voorhees, and Gary J. Fisher.
EGF Receptor_dependent, NFB Independent activation of PI-3 Kinase/Akt Patheay Inhibits
Ultraviolet Irradiation_induced Capases 3, 8, and 9 in Human Kerationcytes. Journal of Bioogicall
Chemistry. 278(46):45737-45 (2003)
13. Taihao Quan*, Tianyuan He*, Sewon Kang, John J. Voorhees, Gary J. Fisher. Solar Ultraviolet
Irradiation Impairs TGF-/Smad Pathway and That This Impairment Contributes Loss of Collagen
Production in Photoaged Human Skin. The American Journal of Pathology 165(3):741-51 (2004)
14. Taihao Quan, Tianyuan He, John J. Voorhees, Gary J. Fisher. Ultraviolet irradiation induces
SMAD7 via induction of transcription factor AP-1 in human skin fibroblasts.
J Biol Chem. 280(9): 8079-85 (2005)
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15. Youpin Deng*, Tianyuan He*, Yinong Wu, Vanka P, Yang G, Huang Y, Yao H, Brown SJ.
Computationally analyzing the possible biological function of YJL103C--an ORF potentially involved
in the regulation of energy process in yeast. Int J Mol Med. 15(1):123-7(2005)
16. Taihao Quan, Tianyuan He, Yuan Shao, Lin Lin, Sewon Kang, John J. Voorhees, Gary J. Fisher
Elevated Cysteine-Rich 61 Mediates Aberrant Collagen Homeostasis in Chronologically Aged and
Photoaged Human Skin The American Journal of Pathology 169(2): 482-90 (2006)
17. Gary J. Fisher, Taihao Quan,Trupta Purohit, Yuan Shao, Moon Kyun Cho, Tianyuan He, James
Varani, Sewon Kang and John J. Voorhees Collagen fragmentation promotes oxidative stress and
elevates matrix metalloproteinase-1 in fibroblasts in aged human skin. The American Journal of
Pathology 174(2): 101-14 (2009)
18. Taihao Quan, Yuan Shao, Tianyuan He, John J. Voorhees, Gary J. Fisher. Reduced expression of
connective tissue growth factor (CTGF/CCN2) mediates collagen loss in chronologically aged human
skin. Journal of Investigative Dermatology 130; 415-24, (2010)
19. Taihao Quan, Zhaoping Qin, Yiru Xu. Tianyuan He, Sewon Kang, John J. Voorhees, Gary J. Fisher.
Ultraviolet irradiation induces CYR61/CCN1, a mediator of collagen homeostasis, through activation
of transcription factor AP-1 in human skin fibroblasts. Journal of Investigative Dermatology 130;
1697-706, (2010)
20. Vicentini FT, He T, Shao Y, Fonseca MJ, Verri WA Jr, Fisher GJ, Xu Y. Quercetin inhibits UV
irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing
NF-B pathway. J Dermatol Sci. 61; 162-8, (2011)
21. Xia W, Hammerberg C, Li Y, He T, Quan T, Voorhees JJ, Fisher GJ. Expression of catalytically
active matrix metalloproteinase-1 in dermal fibroblasts induces collagen fragmentation and functional
alterations that resemble aged human skin. Aging Cell. 2013 Aug;12(4):661-71. [Epub ahead of print]
22. Quan T, Xu Y, Qin Z, Robichaud P, Betcher S, Calderone K, He T, Johnson TM, Voorhees JJ, Fisher
GJ. Elevated YAP and its downstream targets CCN1 and CCN2 in basal cell carcinoma: impact on
keratinocyte proliferation and stromal cell activation. Am J Pathol. 2014 Apr;184(4):937-43 [Epub
2014 Jan 30].
23. He T, Quan T, Shao Y, Voorhees JJ, Fisher GJ. Oxidative exposure impairs TGF-β pathway via
reduction of type II receptor and SMAD3 in human skin fibroblasts. Age. 2014 Jun;36(3):9623. [Epub
2014 Feb 20].
24. He T, Quan T, Fisher GJ. Ultraviolet irradiation represses TGF-β type II receptor transcription
through a 38-bp sequence in the proximal promoter in human skin fibroblasts. Exp Dermatol. 2014
Oct;23 Suppl 1:2-6..
* These authors contributed equally to this work.
Abstracts, Preliminary Communications, Panel Discussions
1.
Tianyuan He, Yaxue Shi, Shishu Chen,. Gene Therapy for Rabbit Hind Limb Ischemia by
Intramuscular Injection of VEGF cDNA. First international symposium on gene therapy Beijing July
7-10,1997.
2.
Xufang Bai, Daosheng Zhang Binyao Wang, TianyuanHe, Shitao Cui, Shishu Chen,. Effects of
VEGF Genes Directly Injected into the Rabbit Heart in vivo. American Society of Gene Therapy 1st
Annual Meeting May 28-31,1998 Seattle, Washington.
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3.
Jiefang Bian, Baigen Zhang, Hushen Qian, Tianyuan He, Zhanyun Tang, Shishu Chen,. Antisense cmyc and Proliferation Nuclear Antigen Oligonucleotide Inhibits Proliferation of Vascular Smooth
Muscle Cells. American Society of Gene Therapy 1st Annual Meeting May 28-31,1998 Seattle,
Washington.
4.
Jiahao Xiao, Tianyuan He, Hs Talwar, Wen Di, EA Duell JJ Voorhees,. Retinoic Acid Receptorbut not Retinoid X Receptor-selective Retinoids Suppress Ultraviolet Light Induction of c-jun
Protein. The Society for Investigative Dermatology, 60th Annual Meeting, May 5-9, 1999 Chicago,
Illinois.
5.
Taihao Quan, Tianyuan He, JJ Voorhees, GJ Fisher Ultraviolet Irradiation Alters Transforming
Growth Factor- and Smad7 Expression in Human Skin in vivo. The Society for Investigative
Dermatology, 61th Annual Meeting, May 10-14, 2000 Chicago, Illinois.
6.
Tianyuan He, Taihao Quan, JJ Voorhees, GJ Fisher,. UV Irradiation Blocks Cellular Responses to
Transforming Growth Factor- Through Downregulation of its Type-II Receptor and Induction of
Smad7. The Society for Investigative Dermatology, 61th Annual Meeting, May 10-14, 2000 Chicago,
Illinois.
7.
GJ Fisher, Taihao Quan, Tianyuan He, JJ Voorhees,. Ultraviolet Irradiation Inhibits Procollagen
Synthesis by Antagonism of Transforming Growth Factor- Signal Transduction in Human Skin in
vivo. Annual Meeting 2000 for the European Society for Dermatological Research Sept. 21-23,2000
Berlin, Germany.
8.
Taihao Quan, Tianyuan He, JJ Voorhees, GJ. Fisher,. UV Irradiation Inhibits Procollagen Synthesis
by Down-regulating the TGF- Type II Receptor in Human Skin Fibroblasts. The Society for
Investigative Dermatology, 62th Annual Meeting, May 9-13, 2001 Washington D.C.
9.
Tianyuan He, Taihao Quan, GJ. Fisher, JJ Voorhees,. Ultraviolet Irradiation Inhibits Expression of
Connective Tissue Growth Factor (CTGF) in Human Skin in vivo and in Human Skin Fibroblasts.
The Society for Investigative Dermatology, 62th Annual Meeting, May9-13, 2001 Washington D.C.
10. Taihao Quan, Tianyuan He, Yuan Shao, JJ Voorhees and GJ. Fisher. Elevated Expression and
Retinoid Regulation of CYR61 in Chronologically-aged, Photoaged, and UV-irradiated Human Skin
in vivo The Society for Investigative Dermatology, 63th Annual Meeting, May 15-18, 2002 Los
Angeles, California.
11. Tianyuan He, Taihao Quan, GJ. Fisher, JJ Voorhees,. Retinoid Induction of Transforming Growth
Factor-1 and Connective Tissue Growth Factor: Major Regulators of Collagen Synthesis, in Aged
and Photoaged Human Skin In Vivo. The Society for Investigative Dermatology, 63th Annual Meeting,
May 15-18, 2002 Los Angeles, California.
12. Taihao Quan, Tianyuan He, JJ Voorhees and GJ. Fisher,. Ultraviolet Irradiation Induction of Smad7
via Activation of Transcription Factor AP-1 Contributes to Loss of Procollagen Gene Expression in
Human Skin Fibroblasts Society for Investigative Dermatology, 64th Annual Meeting, April 30-May
4, 2003 Miami Beach, Florida.
13. GJ. Fisher, Taihao Quan, Tianyuan He, Yuan Shao, JJ Voorhees,. Ultraviolet Irradiation Activates
NF-B Pathway via Association of IB kinase-/NEMO with IB Kinase- and IB- in Human
Skin Keratinocytes Society for Investigative Dermatology, 64th Annual Meeting, April 30-May 4,
2003 Miami Beach, Florida.
14. Tianyuan He, Taihao Quan, Yuan Shao, GJ Fisher, JJ Voorhees,. Cystein-rich Protein (CYR61), A
Mediator of Collagen Homeostasis, Is Elevated in Chronologically Aged, Photoaged, and UV6
irradiated Human Skin in vivo Society for Investigative Dermatology, 64th Annual Meeting, April 30May 4, 2003 Miami Beach, Florida.
15. T He, TH Quan, JJ Voorhees and GJ Fisher,. UV irradiation reduces type I procollagen production by
transcriptional repression through a 90 base pair sequence in the promoter of TGF-; type II receptor
in human skin fibroblasts Society for Investigative Dermatology, 65th Annual Meeting, April 28-May
1, 2004 Providence, Rhode Island.
16. T He, TH Quan, JJ Voorhees and GJ Fisher Acute oxidative stress causes long-lived reduction of
type I collagen and increased collagenase in human skin fibroblasts Society for Investigative
Dermatology, 66th Annual Meeting, May 4-7, 2005 America’s Center, St. Louis, Missouri.
17. T Quan, T He, Y Shao, S Kang, JJ Voorhees and GJ. Fisher,. Elevated cystein-rich protein 61
(CYR61/CCN1) mediates aberrant collagen homeostasis in chronologically-aged and photoaged
human skin. Society for Investigative Dermatology, 67th Annual Meeting, May 3-6, 2006
Philadelphia, Pennsylvania.
18. T He, TH Quan, JJ Voorhees and GJ. Fisher Gene expression profiles of oxidative stress-induced
and replication-induced senescent adult human skin fibroblasts. Society for Investigative
Dermatology, 67th Annual Meeting, May 3-6, 2006 Philadelphia, Pennsylvania.
19. T Quan, T He, Y Shao, Y Xu, S Kong, JJ Voorhees and GJ. Fisher Retinol suppresses
CRY61/CCN1, a negative regulator of collagen homeostasis, in chronologically aged and photoaged
human skin in vivo. International Investigative Dermatology, May 14-17, 2008 Kyoto, Japan.
20. T He, T Quan, Y Shao, JJ Voorhees and GJ. Fisher Oxidative stress reduces collagen production by
impairment of transforming growth factor- signaling in human dermal fibroblasts. International
Investigative Dermatology, May 14-17, 2008 Kyoto, Japan.
21. T He, T Quan, JJ Voorhees and GJ. Fisher Oxidative stress impairs TGF- responsiveness by
coordinate down-regulation of Type II TGF- receptor and smad3 in primary human skin fibroblasts
Society for Investigative Dermatology, May 6-9, 2009 Montreal, Canada.
22. T Quan, Z Qin, Y Xu, T He, JJ Voorhees and GJ. Fisher Ultraviolet irradiation induces
CYR61/CCN1, a novel mediator of collagen homeostasis, via activation of transcription factor AP-1
in human skin fibroblasts Society for Investigative Dermatology, May 6-9, 2009 Montreal, Canada.
23. T He, T Quan, Z Qin, JJ Voorhees and GJ. Fisher UV irradiation reduces Type II TGF- receptor
expression by transcriptional repression through a 38 base pair promoter sequence in human skin
fibroblasts. Society for Investigative Dermatology, May 5-8, 2010 Atlanta, Georgia.
24. Z Qin, T He, JJ Voorhees, GJ. Fisher and T Quan Oxidative stress elevates cystein-rich protein 61
(Cyr61), a novel mediator of photoaged skin , in human skin fibroblasts. Society for Investigative
Dermatology, May 5-8, 2010 Atlanta, Georgia.
25. T Quan, Z Qin, T He, JJ Voorhees and GJ. Fisher Elevated cysteine-rich protein 61 (CYR61)
mediates down-regulation of collagen expression in scenscent human dermal fibroblasts. Society for
Investigative Dermatology, May 4-7, 2011 Phoenix, Arizona.
26. T He, T Quan, Z Qin, JJ Voorhees and GJ. Fisher Induction of microRNA Mir-29 by UV irradiation
down-regulates type I procollagen in fibroblasts in human skin. Society for Investigative
Dermatology, May 4-7, 2011 Phoenix, Arizona.
27. Y Xu, FT Vicentini, T He, Y Shao, MJ Fonseca, WA Verri and GJ. Fisher Quercetin inhibits UV
irradiation induced inflammatory cytokine production in primary human keratinocytes by suppression
NF-kB pathway. Society for Investigative Dermatology, May 4-7, 2011 Phoenix, Arizona.
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28. Gary Fisher, Tianyuan He, Taihao Quan, John Voohees Oxidative stress reduces collagen
production through mTOR-dependent down-regulation of transforming growth factor signaling
pathway in human skin fibroblasts. European Society for Dermatology Research, Sep.7-10, 2011
Barcelona, Spain
29. Tianyuan He, Taihao Quan, Zhaoping Qin, John J Voorhees and Gary J Fisher Induction of
microRNA miR-21 by UV irradiation down-regulates TGF-b type II receptor and type I procollagen
in human skin firboblasts. Society for Investigative Dermatology, May 9-12, 2012 Raleigh, North
Carolina.
30. Tianyuan He, Taihao Quan, Zhaoping Qin, John J Voorhees and Gary J Fisher microRNA-21 and
microRNA-29 act in concert with the TGF-b pathway to sown-regulate synthesis of type I collagen in
response to UV irradiation in human skin fibroblasts. Society for Investigative Dermatology, May 710, 2014 Albuquerque, New Mexico
BOOK CHAPTERS
Tianyuan He, Binkun Jiang, The Chemistry of Nucleic Acid. Yong Wei Eds. In Medical
Biochemistry. World Book Press Company, P.R. China. 1995 p75-90.
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