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Hybrid Multi-Mode Microplate Reader
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3 Multi-Volume Multi-Sample Plate with 2 µL
microspots
 Specifications and Summary
Synergy™ H4: Hybrid Technology
Filter-based optics:
 Use of dichroic mirrors increases
sensitivity about 5x compared to
filter-only optics
 Peak transmission for filters and
mirrors is around 80%. Very
efficient optical system
 Because of high amount of
energy delivered to samples,
high sensitivity can be obtained
with short read times
 In summary, a fast and very
sensitive optical system
Synergy™ H4: Hybrid Technology
Monochromator-based optics:
 Use of two gratings per
monochromator provides
smooth performance from 250
to 900 nm
 Very large range of
applications, including spectral
scanning
 Variable bandpass system
adds flexibility
 In summary, an extremely
flexible optical system
Synergy™ H4: Hybrid Technology
Filter
Monochromator
%CV
Assay Window
Z'
Patent-pending design:
 Flexible monochromator system
 High-performance filter system
 Each with its strengths and
weaknesses
 Extremely versatile instrument
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
High-performance, easy-to-use filter system
Dichroic mirrors:
 Easy side access
 Easy load/unload
software function
Filter wheels:
 Easy front access
 No tool required
 Easy load/unload software function
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Automated Z-Focusing
Top fiber
300 µl
200 µl
100 µl
If no Z-focusing available,
performance issues in low volumes,
or use of adapters required.
300 µl
200 µl
100 µl
With Z-focusing, reading height can
be precisely adjusted (100 µm
resolution) for best performance on
all plate types, and with all
volumes.
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Dual Reagent Dispenser Option
35000
Fluorescence (RFU)
30000
25000
20000
15000
10000
5000
0:00:00
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0:01:00
0:02:00
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0:04:00
0:05:00
Time (minutes)
Two reagent dispensers
Baseline
Response
Volume range 5 µL - 1000 µL
Enables dispense/read processes
Trigger Reagent
Ion channel assays, flash
Injection
luminescent assays, time-sensitive
kinetic assays
0:06:00
0:07:00
0:08:00
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Advanced 4-ZoneTM Temperature Control System
 4 independent heaters with 4
monitoring sensors
 Precision: +/- 0.5°C at 37°C
 Range: RT to 65°C
 Enables applications with strict
temperature uniformity
requirements (e.g. ORAC
assays)
 Enables high-temperature
assays (e.g. Invader assays at
62°C)
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4
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Take3™ Multi-Volume Multi-Sample Plate with 2 µL microspots
 Sixteen 2 µL microspots
 Enables low volume A260 nucleic
acid quantification
 dsDNA detection limit 2 ng/ µL
 Can also be used for A280
protein quantification, as well as
fluorescence or luminescence
detection
Contents
 Synergy H4 – Hybrid Technology
 Key Features and Benefits
 Quadruple Grating Architecture
 Variable Band-Pass Selection
 High-Performance, Easy-to-Use Filter System
 Automated Z-Focusing
 Dual Reagent Dispenser Option
 Temperature Control System to 65°C
 Take3™ Multi-Volume Plate with 2 µL microspots
 Specifications and Summary
Summary: Versatility Redefined
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Hybrid Technology: Monochromators and Filters
Quadruple Grating Optical Architecture
Variable Bandpass Selection
Dichroic Mirrors and Filters for High Performance
Dual Reagent Dispenser Option
Temperature Control System to 65°C
Take 3™ Multi-Volume Plate with 2 µL microspots
Visit www.biotek.com for more information