Download Neurons and Modified CMOS Integrated Circuits Interfacing

NEUMIC
Project No. IST-1999-29091
Neurons and Modified CMOS Integrated
Circuits Interfacing
Imec
The Hebrew University of Jerusalem
Physics, Chemistry,Biology
Project funded by the Future and Emerging Technologies arm of the IST Programme
Neuro-Electronic Synapse (NES):
interact with neuronal communication
action potential
neurotransmitter
© KdK2002
eNES
bi-directional electrical
communication
cNES
uni-directional chemical
communication
Project funded by the Future and Emerging Technologies arm of the IST Programme
Neurons on a chip
Project funded by the Future and Emerging Technologies arm of the IST Programme
RAW
FILTERED (100-1500Hz)
V [mV]
V [mV]
FG tor output
Recorded action potential
time [sec]
V [102uV]
V [102uV]
intracellular
voltage
time [sec]
time [sec]
time [sec]
Project funded by the Future and Emerging Technologies arm of the IST Programme
A hybrid transducer
for molecular recognition events
neuronal pre-synaptic element
“artificial
acetylcholine synapse”
molecular
recognition
event
modulated
current
acetylcholine
anti-ACh
ACh-esterase
modulated current
electronic post-synaptic element
synapse GF
Project funded by the Future and Emerging Technologies arm of the IST Programme
cNES: towards an artificial synapse
AcetylCholine-Esterase ISFET
(A)
Without enzyme layer
14
12
10
8
6
4
2
0
7
6
5
4
Ids (uAmp)
With enzyme layer
3
-log([Ach])
ACh
(B)
Ids (Amp)
no acetylcholine
-0.20
7.00E-05
6.00E-05
5.00E-05
4.00E-05
3.00E-05
2.00E-05
1.00E-05
0.00E+00
-0.10
0.00
0.10
10uM acetylcholine
0.20
0.30
0.40
0.50
0.60
Vgs (Volt)
SAM of AChE
acetic acid
fast (35 sec) & sensitive (10-8 M)
Project funded by the Future and Emerging Technologies arm of the IST Programme
Achievements so far, and future
•Fabrication of 1st generation experimental Si neuron transistor chip
(single neurons, extended floating gate, depletion type,shielding metals,
SiO2 Ta2O5)
•New package designed and realized
(flip chip bumping, large exposure of Si area, easy access microscope,
microprobes..)
•Recorded signals from stimulated neurons cultivated on chip
•Experimental verification of “Molecular amplifiers” based on
chromophores
•Improved neuron adhesion layer, next to polylysine
•Assessment and verification of chemical sensing principle by direct and
quantized measurements of ACh
•Second generation transistors designed, processing start end of December
(improved S/N, improved processing, multiple neurons, bi-directional…)
•Selection of improved surface biologization molecules and patterning
•Direct measurement of ACh on chip
•On chip stimulation and recording of neurons
Project funded by the Future and Emerging Technologies arm of the IST Programme
Conclusion
No major delays on deliverables
Interactiva’s tasks have been divided between partners
Expected successful completion of 3th year
if extension till December 2003, no show stopper yet
Second run and purchase of peptides, sacharides necessary
and possible after administrative problems are solved,
expected December 2002
Agreement from project officer obtained summer 2002
Project funded by the Future and Emerging Technologies arm of the IST Programme
Changes to the workplan
Second generation transistors
Surface biologization
Technology implementation plan
Project funded by the Future and Emerging Technologies arm of the IST Programme