Download Name: Ms. Marguerite McDonald Title: Biochemist, grad student Concentration: Enzymology Work Place :

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Name: Ms. Marguerite McDonald
Title: Biochemist, grad student
Concentration: Enzymology
Work Place :
Dr. Mueller’s
Mechanistic Enzymology Lab
Chemistry and Biochemistry Department
University of Delaware Graduate Program
Biochemistry
DNA
RNA
PROTEIN
http://bioweb.wku.edu/courses/BIOL115/Wyatt/Nucleic_Acids/mRNA2.htm
Mechanistic Enzymology
The study of the action (the mechanism) of an enzyme
Ψs
ynt
has
e
Pseudouridine synthases
http://www.sdnhm.org/exhibits/epidemic/teachers/images/bacteria.jpg
The Chemistry:
Isomerization of RNA
TruA’s substrate: transfer RNA
Purifying radioactive tRNA
and TruA
HPLC trace and radioactivity/min
450000
HPLC trace
dpms/10 ul fraction
400000
350000
dpm
300000
250000
200000
150000
100000
50000
0
0
5
10
15
20
25
time
SDS-polyacrylamide gel showing pure
TruA and TruA before purification
urea-polyacrylamide gel showing pure tRNA and
tRNA with DNA contamination
Pseudouridine Synthases
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