Download Colonies and Stains PPT

Cocci
Bacilli
Spirochetes
Staphylococcus Arrangement
Diplococcus
Streptococcus
Streptobacillus Arrangement
Whole Colony
round
irregular
filamentous
rhizoid
MARGIN
Smooth,
entire
rhizoid
Irregular
(erose)
filamentous
lobate
ELEVATION
convex
raised
umbonate
raised,
spreading edge
plateau
flat
flat raised
margin
growth into
medium
Opaque Center
and Spreading Edge
Shiny and Umbonate
Irregular and Rhizoid
Punctiform Colonies
Stains
Wet Mount (Hanging Drop)
Cheek Cells Without Stain
Cheek Cells with Simple Stain
(Methylene Blue)
Simple Stain: Negative Stain
Negative Stain
Differential Stain: Gram Stain
Primary stain:
Crystal violet
Mordant:
Iodine
Decolorizing agent:
Alcohol-acetone
Counterstain:
Safranin
Color of
Gram +
Purple
Color of
Gram –
Purple
Purple
Purple
Purple
Colorless
Purple
Pink
Outer membrane
Peptidoglycan
GRAM NEGATIVE
GRAM POSITIVE
Gram Positive and Gram Negative
Bacteria
Gram Stain
of Staphylococcus epidermidis (+)
and Citrobacter diversus (-).
Acid-Fast Stain
ZN Method
Acid-Fast and Non Acid-Fast
Endospore Stains
Endospores
Endospore Stain: Ziehl-Neelsen
Figure 3.12a-c
Endospore Stain
Stain Comparisons
G+
(PURPLE)
G–
(PINK)
ACID
FAST
ENDOSPORE
STAIN
PRIMARY STAIN
Crystal violet Crystal violet Carbol
Fuschia
Malachite
green
MORDANT
Iodine
Heat
Heat
DECOLORIZER
ETOH or
ETOH or
acetone:
acetone:
Cell is purple Cell is clear
Acid
alcohol
Water
Iodine
COUNTER-STAIN Safranin:
Safranin:
Cell is purple Cell is pink
Methylene Safranin:
Blue
Spores are
green
Cell is pink
Capsule Stain
Capsule Stain
Figure 3.12a-c
Flagella Stain
Flagella Stain
Peritrichous Flagella
Figure 3.12a-c
Names for flagella arrangement
• Monotrichous
• Lophotrichous
• Amphitrichous
• Peritrichous