Download Stains - Dr Magrann

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

page
1
page
2
page
3
page
4
page
5
page
6
page
7
page
8
page
9
page
10
page
11
page
12
page
13
page
14
page
15
page
16
page
17
page
18
Document related concepts
no text concepts found
Transcript 
Stains
Negative Stain
Cheek Cells Without Stain
Cheek Cells with Methylene Blue
Differential Stains: Gram Stain
Primary stain:
Crystal violet
Mordant:
Iodine
Decolorizing agent:
Alcohol-acetone
Counterstain:
Safranin
Color of
Gram +
Purple
Color of
Gram –
Purple
Purple
Purple
Purple
Colorless
Purple
Pink
Gram Positive and Gram Negative
Bacteria
Outer membrane
Peptidoglycan
GRAM NEGATIVE
GRAM POSITIVE
Capsule Stain
Capsule Stain
Figure 3.12a-c
Non Acid-Fast(A) and Acid-Fast (B)
Endospore Stain: Ziehl-Neelsen
Figure 3.12a-c
Endospore Stain
Flagella Stain
Flagella Stain
Figure 3.12a-c
Stain Comparisons
G+
(PURPLE)
G–
(PINK)
ACID
FAST
SPORE
STAIN
PRIMARY STAIN
Crystal violet Crystal violet Carbol
Fuschia
Malachite
green
MORDANT
Iodine
Heat
Heat
DECOLORIZER
ETOH or
ETOH or
acetone:
acetone:
Cell is purple Cell is clear
Acid
alcohol
Water
Iodine
COUNTER-STAIN Safranin:
Safranin:
Cell is purple Cell is pink
Methylene Safranin:
Blue
Spores are
green
Cell is pink
Related documents