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Gene Regulation Prokaryotes (no nucleus) Lac operon • System of genes that can turn on and off in E. coli (bacteria). • These genes make enzymes that break down the sugar in milk (lactose). • Regulatory genes~ genes that affect other genes (make them turn on or off) • Structural genes~genes that code for a protein Structure of the Lac operon Regulatory genes S g #3 S g #2 Inhibitor/repressor (gene) Promoter “region” (gene) Operator “region” (gene) Structural gene #1 Function of the Lac operon Regulatory genes S g #3 S g #2 Inhibitor/repressor (gene) Promoter “region” (gene) Operator “region” (gene) Structural gene #1 Proteins that break down lactose Repressor (protein) inhibitor ? promoter Lactose operator SG 1 SG2 RNA polymerase SG3