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Transcript 
Carbon Monoxide Dehydrogenase Activity
in Bradyrhizobim japonicum
이정은
Introduction
• Carbon monoxide (CO) dehydrogenases (CODHs)
- a key enzyme in the CO metabolism
- carboxidotrophic bacteria
- catalyzes the oxidation of CO to CO2
CO + H2O -> CO2 +2e- +2H+
- contains 1) molybdopterin
2) [2Fe – 2S]
•
-
Bradyrhizobium japonicum species
gram-negative soil bacteria
N2- fixing symbiosis
utilize H2 and CO2 as a sole sources of energy and carbon
use CO as a a sole sources of energy and carbon
Results
CO- dependent growth
Purification and properties of CODH
Metal contents and analysis of flavins and
carboxamidomethylated proteins
Discussion
•
B. japonicum 110spc4
-
grew under CO-autotrophic condition.
hydrogen bacteria
utilize H2 and CO2 as a sole sources of energy and carbon
use CO as a a sole sources of energy and carbon
contain a CODH enzyme
• CODH in 110 spc4
- NBT/ PMS
- value of CO-oxidizing activity
- nitrate reductase activity
- heterohexameric subunit (LMS)2
- 75-kDa polypeptide /Cox L subunit of the O.carboxydovorans
(immunological relation)
•
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