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27. 07. 2011
Student: Jan Česal
Leader: Václav Šlouf
Effect of the Solvent Environment
on Excited-State Properties of an Untypical
Carotenoid
Motivation
-
earlier measurements on LH1-RC of AAP
(Aerobic Anoxygenic Phototrophic) bacteria
S2
Hu et al., PNAS 1998
2
Qx
ET
S1
Qy
1
S0
Bacteriorubixanthinal
1
c

1
 ET

Bacteriochlorophyll
S0
1
c = 0.6 ps
1
1 = ... will be the result of this study
ET = ?
Materials and methods
Probe
Pump–probe spectroscopy
Delay line
- 110 fs
- 1 kHz
Crystal
Sample
Laser system
Pump
Light converter
Spectrograph
Materials and methods 2
• Extraction of Bacteriorubixanthinal (Brx)
- HPLC (High-performance liquid chromatography)
[V]
[%]
C:\data_prep\LCdata\Kata\BRX11-07-15-C - INT7 - 1
9
19
23,3
1,0
9,8
1,2
80
18
8
22,4
7
9,2
34
35
43,5
33
39,7
21
25,4
0,0
42,3
31
32
36,8
37,6
30
29
34,6
28
32,1
33,7
26
27
31,0
25
29,2
29,9
22
23
24
27,1
27,6
20
40
28,4
17
16
18,0
19,5
15
17,0
14
11
15,5
10
13,3
14,2 12
14,7 13
12,7
1
2,8
3,9
3
0,2
5,5
3,2
4
2
7,0
0,4
24,2
5
8,0
6
8,5
60
0,6
Components
Voltage
0,8
20
0
0
10
20
30
Time
40
[min.]
http://en.wikipedia.org/wiki/File:Agilent1200HPLC.jpg
Results
Steady-state spectroscopy
500nm
Transient absorption
spectra
Kinetics
1  3.4 ps
Conclusions
- We measured 1
- We calculated ET
1
c

1
 ET
S2
ET = 0.73 ps
S1
Brx
1
Qx
Qy
1 = 3.4 ps
S0

1
BChl
S0
Thanks for your attention