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Lesson 1.2 Suspect Profiling Definitions and templates for: Case Notes 1.2 l Investigation Activity 1.2 Viral Types Penicillium spp. Escherichia coli O157:H7 Saccharomyces cerevisiae Definition Bacteria • Single-cell microorganism with no clearly defined nucleus (prokaryote), capable of self-replication. • Bacteria usually reproduce by one cell splitting in two. • With ideal conditions they split every half hour. In 12 hours one cell could multiply into 33 million cells! Definition Bacteria • Bacteria can use most carbohydrates, fats, and proteins as sources of nutrients. • Because of this you can find them nearly everywhere • In air, soil, and water • In intestines of animals and humans • On raw meat, poultry, and seafood Definition Bacteria can also be on • Skins of fruits and vegetables • Shells of nuts • Insects and rodents • Hand, skin, hair, and clothing of people Definition Bacteria Some bacteria have the ability to form seed like structures that allows them to survive for longer periods of time under inhospitable conditions. This form is called a spore. Definition Virus • A piece of nucleic acid (DNA or RNA) surrounded by a protein membrane. • A virus cannot reproduce on its own. It must infect the cell of another living organism before it can replicate. Definition Fungi A kingdom of organisms that include mushrooms, molds, and yeasts. Definition Two types of fungi commonly associated with food microbiology are • Mold • Yeast Saccharomyces cerevisiae Penicillium spp. Definition Mold • Multi-cellular, filamentous fungi that grow very quickly by asexual reproduction. • Need little water. • Do need the right temperature and level of oxygen to grow. • Prefer room or higher temperatures. Definition Yeast • Unicellular fungi that usually go through cell division by budding. • Although they are unicellular (single cell) organisms, yeasts commonly grow together in groups to break down sugar and starches. • Yeasts require moist or wet environments. Definition Parasite An organism that lives on or in another organism often at the expense of the host organism. Pigs eating raw garbage Raw or inadequate cooking Giardia on intestinal wall (Image by S. Wade) Suspect Profiling Case Notes 1.2 Name:_____________________________ Date:___________________________ Why is Criminal Profiling used in investigations? ______________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ Microorganism 1. 2. 3. 3a. 3b. 4. Definition Case Notes 1.2 Suspect Profiling Investigation Activity 1.2 Name: ____________________________ Date: ___________________________ When investigators profile likely suspects they look at large segments of the population and collect data on the backgrounds, characteristics, behaviors, and traits of various types of suspects. Directions: Use the material provided, including the booklet accompanying the prepared slides, to create a profile for each category of likely suspects in our FSI foodborne illness outbreak investigation. First – Sketch what you see under the microscope on each slide (Note: for virus use the PPT 1.2 image). Second – Using the references provided, label your sketch with the structures of the organisms and describe 2-3 of the defining characteristics in the column on the right. Suspect Category 1 Bacteria List 2-3 Characteristics Suspect Category 2 Viruses List 2-3 Characteristics Investigation Activity 1.2 Suspect Category 3 Parasites List 2-3 Characteristics Suspect Category 4 Yeasts List 2-3 Characteristics Suspect Category 5 Molds List 2-3 Characteristics Investigation Activity 1.2 (cont.) Escherichia coli O157:H7 Viral Types Giardia lamblia Penicillium spp. Scanning Electron Micrograph of Saccharomyces cerevisiae National GAPs Program © 2007 Department of Food Science Department of Education Cornell University Ithaca, NY 14853 www.gaps.cornell.edu