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Transcript 
William S. Klug
Michael R. Cummings
Charlotte A. Spencer
Concepts of Genetics
Eighth Edition
Chapter 6
Genetic Analysis and Mapping
in Bacteria and Bacteriophages
Copyright © 2006 Pearson Prentice Hall, Inc.
Bacteria Mutate
Spontaneously and Grow at
an Exponential Rate
Figure 6-1
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-2
Copyright © 2006 Pearson Prentice Hall, Inc.
Conjugation Is One Means of
Genetic Recombination in
Bacteria
Figure 6-3
Copyright © 2006 Pearson Prentice Hall, Inc.
Conjugation Is One Means of
Genetic Recombination in
Bacteria
F+ and F- Bacteria
Figure 6-4
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-5
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-6
Copyright © 2006 Pearson Prentice Hall, Inc.
Conjugation Is One Means of
Genetic Recombination in
Bacteria
Hfr Bacteria and Chromosome Mapping
Figure 6-7
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-8
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-9
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-9a
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-9b
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-10
Copyright © 2006 Pearson Prentice Hall, Inc.
Conjugation Is One Means of
Genetic Recombination in
Bacteria
Recombination in F+ X F- Matings:
A Reexamination
The F' State and Merozygotes
Figure 6-11
Copyright © 2006 Pearson Prentice Hall, Inc.
Mutational Analysis Led to
the Discovery of the Rec
Proteins Essential to
Bacterial Recombination
F Factors Are Plasmids
Figure 6-12
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-12a
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-12b
Copyright © 2006 Pearson Prentice Hall, Inc.
Transformation Is Another
Process Leading to Genetic
Recombination in Bacteria
The Transformation Process
Figure 6-13
Copyright © 2006 Pearson Prentice Hall, Inc.
Transformation Is Another
Process Leading to Genetic
Recombination in Bacteria
Transformation and Linked Genes
Bacteriophages Are Bacterial
Viruses
Phage T4: Structure and Life Cycle
Figure 6-14
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-15
Copyright © 2006 Pearson Prentice Hall, Inc.
Bacteriophages Are Bacterial
Viruses
Lysogeny
Transduction Is VirusMediated Bacterial DNA
Transfer
The Lederberg–Zinder Experiment
Figure 6-17
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-18
Copyright © 2006 Pearson Prentice Hall, Inc.
Transduction Is VirusMediated Bacterial DNA
Transfer
The Nature of Transduction
Transduction and Mapping
Bacteriophages Undergo
Intergenic Recombination
Figure 6-16
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-19
Copyright © 2006 Pearson Prentice Hall, Inc.
Table 6-1
Copyright © 2006 Pearson Prentice Hall, Inc.
Bacteriophages Undergo
Intergenic Recombination
Mapping in Bacteriophages
Table 6-2
Copyright © 2006 Pearson Prentice Hall, Inc.
Intragenic Recombination
Occurs in Phage T4
The rII Locus of Phage T4
Figure 6-20
Copyright © 2006 Pearson Prentice Hall, Inc.
Intragenic Recombination
Occurs in Phage T4
Complementation by rII Mutations
Figure 6-21a
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-21b
Copyright © 2006 Pearson Prentice Hall, Inc.
Intragenic Recombination
Occurs in Phage T4
Recombinational Analysis
Figure 6-22
Copyright © 2006 Pearson Prentice Hall, Inc.
Intragenic Recombination
Occurs in Phage T4
Deletion Testing of the rII Locus
Figure 6-23
Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 6-24
Copyright © 2006 Pearson Prentice Hall, Inc.
Intragenic Recombination
Occurs in Phage T4
The rII Gene Map
Figure 6-25
Copyright © 2006 Pearson Prentice Hall, Inc.
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