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Transcript 
LUCIFERASE
A. Cengiz Öztireli
BIOLUMINESCENCE
BIOLUMINESCENCE (LIVING LIGHT)
Milky Sea
BIOLUMINESCENCE
Applications
BIOLUMINESCENCE
Bacteria
Sea Anemones
Dinoflagellates
BIOLUMINESCENCE
FIREFLYES (PHOTINUS)
Attracting mates
Attracting prey
LUCIFERASE
FIREFLY
BACTERIA
BIOLUMINESCENCE
8 ATP → 1 Green Photon
Reaction:
Luciferin
Luciferase + O2
ATP
Inter.
P*
P + hv
BIOLUMINESCENCE
Color
 P*
 P* + F → F* + P
 Proteins around
 Structure
PHOTINUS PYRALIS (FIREFLY)
PHOTINUS PYRALIS LUCIFERASE
Luciferase + Luc-COOH
ATP
Luciferase: Luc-CO-AMP
O2
Luciferase: Luc=O*
PHOTINUS PYRALIS LUCIFERASE
Structure and Functioning
C-Terminal
Cleft
N-Terminal
CONCLUSIONS
THANK YOU
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