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Contents
Abbreviations
How to use this book
Preface
1. Biological Heredity and Variation
Mendelian inheritance
Segregation at one locus
Segregation at two loci
Quantitative inheritance
2.
The Cell Cycle
The bacterial cell cycle
The eukaryotic cell cycle
The molecular basis of cell cycleregulation
Progress through the cell cycle
Special cell cyclesystems in animals
3. Chromatin
Nucleosomes
Higher order chromatin organization
Chromatin and chromosome function
Molecular structure of the bacterial nucleoid
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Chromosome Mutation
Numerical chromosome mutations
Structural chromosome mutations
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5. Chromosome Structure and Function
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Normal chromosomes - gross morphology
Special chromosome structures
Molecular aspects of chromosome structure
6. Development, Molecular Aspects
Differentiation
Pattern formation and positional information
The environment in development
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7. DNA Methylation and Epigenetic Regulation
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DNA methylation in prokaryotes
DNA methylation in eukaryotes
Epigenetic gene regulation by DNA methylation in mammals
8. The Gene
The concept of the gene
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Units of genetic structure and genetic function
Gene-cistron relationship in prokaryotes and eukaryotes
Gene structure and architecture
9. Gene Expression and Regulation
Gene expression
Gene regulation
Gene expression in prokaryotes and eukaryotes
10. Gene Transfer in Bacteria
Conjugation
Transformation
Transduction
11. The Genetic Code
An overview of the genetic code
Translation
Special properties of the code
12. Genomes and Mapping
Genomes, ploidy and chromosome number
Physico-chemical properties of the genome
Genome size and sequence components
Gene structure and higher-order genome organization
Repetitive DNA
Isochore organization of the mammalian genome
Gene mapping
Genetic mapping
Physical mapping
13. Mobile Genetic Elements
Mechanisms of transposition
Consequences of transposition
Transposons
Retroelements
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14. Mutagenesis and
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15. Mutation and Selection
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DNA Repair
Mutagenesis andreplication fidelity
DNA damage: mutation andkilling
DNA repair
Direct reversalrepair
Excision repair
Mismatch repair
Recombination repair
The SOS response andmutagenic repair
Structural and functional consequences of mutation
Mutant alleles and the molecular basis of phenotype
The distribution of mutations andmolecular evolution
Mutations in Genetic Analysis
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Contents
16.
Nucleic
Nucleic
Nucleic
Nucleic
Acid Structure
acid primary structure
acid secondary structure
acid tertiary structure
17.
Nucleic Acid-Binding Properties
Nucleic acid recognition byproteins
DNA-binding motifs in proteins
RNA-binding motifsin proteins
Molecular aspects of protein-nucleic acidbinding
Sequence-specific binding
Techniques forthe study of protein-nucleic acidinteractions
18. Oncogenes and Cancer
Oncogenes
Tumor-suppressor genes
19. Organelle Genomes
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Organelle genetics
Organelle genomes
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Plasmids
Plasmid classification
Plasmid replication and maintenance
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21. The Polymerase Chain Reaction (PCR)
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Specificity of the PCR reaction
Advances andextensions to basicPCR strategy
Alternative methods for in vitro amplification
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Proteins: Structure, Function and Evolution
Protein primary structure
Higher order protein structure
Protein modification
Protein families
Global analysis of protein function
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Protein Synthesis
The components of protein synthesis
The mechanismof protein synthesis
The regulation of protein synthesis
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Recombinant DNA and Molecular Cloning
Molecular cloning
Strategies for gene isolation
Characterization of cloned DNA
Expression of cloned DNA
Analysis of gene regulation
Analysis of proteins and protein-protein interactions
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In vitro mutagenesis
Transgenesis: gene transfer to animals andplants
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Recombination
Homologous recombination
Homologous recombination and genetic mapping
Random and programmednonreciprocal recombination
Site specific recombination
Generation of immunoglobulin andT-cell receptor diversity
Illegitimate recombination
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Replication
Replication strategy
The cellular replisomeand the enzymology of elongation
Initiation of replication
Primers and priming
Termination of replication
The regulation of replication
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RNAProcessing
Maturation of untranslated RNAs
End-modification and methylation of mRNA
RNA splicing
RNA editing
Post-processing regulation
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SignalTransduction
Receptors and signaling pathways
Intracellular enzyme cascades
Second messengers
Signal ddivery
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Transcription
Principles of transcription
Transcriptional initiation in prokaryotes -basal and constitutive components
Transcriptional initiation in eukaryotes -basal and constitutive components
Transcriptional initiation -regulatory components
Strategies fortranscriptional regulation in bacteria and eukaryotes
Transcriptional elongation and termination
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30. Viruses and Subviral Agents
Viral infection strategy
Diversity of replication strategy
Strategies for viralgene expression
Subviral agents
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Index
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