* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Worksheet for Morgan/Carter Laboratory #9 Drosophila
History of genetic engineering wikipedia , lookup
Population genetics wikipedia , lookup
Fetal origins hypothesis wikipedia , lookup
Genetic engineering wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Neuronal ceroid lipofuscinosis wikipedia , lookup
Saethre–Chotzen syndrome wikipedia , lookup
Gene expression profiling wikipedia , lookup
Point mutation wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Quantitative trait locus wikipedia , lookup
Gene desert wikipedia , lookup
Genome (book) wikipedia , lookup
Gene therapy wikipedia , lookup
Helitron (biology) wikipedia , lookup
Gene therapy of the human retina wikipedia , lookup
Dominance (genetics) wikipedia , lookup
Hardy–Weinberg principle wikipedia , lookup
Gene expression programming wikipedia , lookup
Gene nomenclature wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Worksheet for Morgan/Carter Laboratory #9 “Mendelian Genetics II: Drosophila” Ex. 9-1: ESTABLISHING THE ENZYME REACTION CONTROLS Propose a hypothesis about AO activity in flies from vial 1a and flies from vial 1b. Predict the results of the experiment (test) based on your hypothesis (if/then). Why is it necessary to homogenize the flies? Record your results in the table below: Vial 1a (AO present) Fly # Sex (F or M) AO Activity (+/-) Vial 1b (AO absent) Fly # 1 1 2 2 3 3 4 4 5 5 6 6 Sex (F or M) AO Activity (+/-) Do your results match your predictions? Does the sex of the fly appear to have an impact on the results of the assay test? Which two characteristics are most useful to your group in determining the sex of the flies? Ex. 9-2: DETERMINING THE PATTERN OF INHERITANCE OF THE ALDOX GENE What is the genotype of the female (maternal parent lacks the enzyme) in our cross? What would be the genotype of the male (paternal parent has the enzyme) in our cross if the gene is not sex-linked? What would be the genotype of the male if the gene is sex-linked? What would be the genotypes of the F1 progeny if the gene is not sex-linked? What would be the genotypes if the gene is sex-linked? Propose a hypothesis about the inheritance of this gene. Is it sex-linked? According to your hypothesis, what will be the genotypes of the parents? Predict the offspring from the hypothesized parents (hint: use a Punnett square) Record your results in the table below: Fly # Sex (F or M) AO Activity (+/-) Positive control: _________________________ Fly # 1 11 2 12 3 13 4 14 5 15 6 16 7 17 8 18 9 19 10 20 Sex (F or M) AO Activity (+/-) What allele appears to be dominant? What evidence of your results above supports your answer? Based on your results, should the aldox gene be written beginning with a capital or lowercase letter? Correctly write the names of the wild-type and mutant alleles. By convention, wild-type alleles are designated with a superscript + after the name (e.g., gene+). Did you hypothesize that the gene was sex-linked (on a sex chromosome) or autosomal? Describe how your results either support or falsify your hypothesis. Write a statement describing your conclusions from this experiment. Ex. 9-3: DETERMINING PARENTAL GENOTYPES USING EVIDENCE FROM PROGENY Record your results in the table below: Fly # Positive control: _________________________ AO Activity (+/-) Fly # 1 13 2 14 3 15 4 16 5 17 6 18 7 19 8 20 9 21 10 22 11 23 12 24 AO Activity (+/-) Total the number of offspring in each phenotype category below: Your Totals AO-present AO-absent Is the trait sex-linked? Which allele is dominant? Class Totals Using all observations, hypothesize the genotypes of the parent flies, making sure to name the alleles correctly. Predict the results of the experiment (if/then). Do the class results support or falsify your hypothesis? Does the experiment support or contradict your conclusions concerning the pattern of inheritance derived from Experiment 9.2? REVIEWING YOUR KNOWLEDGE List the most obvious characteristics used to determine the sex of a fruit fly. A fruit fly geneticist discovered a genetic mutation that resulted in pupae and young flies with dark pigment granules in the nuclei and cytoplasm of their fat cells. After studying the inheritance of the mutation, the geneticist named the gene Frd (for Freckled). What does this name tell you about the inheritance of this gene? In another strain of fruit flies, geneticists discovered a mutation producing flies with no gut muscles. They named the gene controlling this phenotype jeb (for jelly belly). What does this tell you about the inheritance of this gene? Is the genetic map or linkage map produced by determining recombination frequencies an actual physical map of the chromosome? Refer to your textbook.