Download Screening Preferentially Expressed Genes in Plumbago Sperm Cells

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Transcript 
Male gamete development
Meiosis
Mitosis I
Mitosis II
Pollen
Microsporocyte
Tetrad
Generative
cell
Vegetative
cell
Sperm cells
Egg cell
Meiosis
Germinated
pollen
Female
Central cell
egg + sperm cell
central cell + sperm
embryo
endosperm
Double fertilization
Posters here at BSA
• Recent Topics #132
– Sperm gene expression
• Recent Topics #133
– Plumbago regeneration and transformation
system
Heterospermy
If sperm cells differ during their origin at generative cell
division, the sperm cells may differ:
•Nuclear heterospermy: non-disjunct chromosomes
at sperm formation (e.g., B-chromosomes in maize)
•Cytoplasmic heterospermy: differential organelle
apportionment at sperm cell formation
Model:
Plumbago
zeylanica
Functional Groups
Functional categories of differentially expressed sperm genes
Functional Categorization of SSH ESTs
Sua
• Transcription, translation, post-translational
modification, protein catabolism
Functional Categorization of SSH ESTs
Svn
• Biosynthesis, energy production, carohydrate
transport, cell transport
Differential Svn–Expressed Genes
• Polyubiquitin
ubiquitin: E1, E2, E3,
26S proteosome
• Isopentenyl
transferase
(cytokinin synthase
control enzyme)
• Energy-related
products
Plumbago Svn-IPT promoter in transgenic
Arabidopsis
Acknowledgements
• OU-Norman
• Sequencing (ACGT)
• Uni Melbourne
• Microarray Facility
–
–
–
–
–
–
–
–
–
Xiaoping Gou
Tong Yuan
Xiaoping Wei
Zhaojie Zhang*
Mohan Singh
Prem Bhalla
Huiling Xu*
Ines Swoboda*
Takashi Okada*
*past participants
–
–
–
–
Bruce Roe
Doris Kupfer*
Yuhong Tang
Sunkyoung So
– Tyrrell Conway
– Mary Beth Langer*
• Funding
– University of Oklahoma
• Web Access
http://www.genome.ou.edu/
Sperm EST Overview
Sua (female target: egg cell/nucleus) 
583 unique EST sequences
– 191 contigs and 392 singlets // 427 are different from Sua
– 9 ribosomal sequences, 18 plastid sequences and 2 mitochondrial
sequences
– 20 nuclear-expressed plastid sequences, 2 mitochondrial sequences
Svn (female target: central cell/polar nuclei):
471 unique EST sequences
– 122 contigs and 349 singlets // 330 are different from Svn
– 6 ribosomal sequences, 3 plastid sequences and 9 mitochondrial
sequences
– 3 nuclear-expressed plastid sequences, 6 mitochondrial sequences
About 70 ESTs are identical (< E-160) and 40 are highly
related (between E-160 and E-060)
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