Download Gallé Ágnes - Antioxidant responses during fusarium infection

Antioxidant responses during fusarium infection: the
sampling and the first results
Gallé Ágnes
Fusarium head blight (FHB) is an important disease of wheat
and has worldwide distribution, which results in yield loss
and mycotoxin contamination of grain when the disease is
caused by a mycotoxin-producing Fusarium species.
Fusarium graminearum and F. culmorum are the Fusarium
species that most commonly cause FHB of wheat and both
can produce trichothecene mycotoxins, predominantly
deoxynivalenol (DON, also known as vomitoxin) (Parry et
al. 1995).
The fungus produces trichothecene mycotoxins, such as
deoxynivalenol (DON).
Effect:
• livestock feed refusal
• depression of the immune system
• nausea and vomiting in humans
(Hall and Van Sanford, 2003)
Resistance to FHB is very complex and is considered to be
quantitatively governed by numerous resistance genes located on
different chromosomes.
At least 18 of the 21 wheat chromosomes have been reported
to be associated with resistance.
The components of the defense reactions have been extensively
investigated with both protein and transcript profiling.
Some gene families which were induced during Fusarium infection (microarray):
Saber et al 2007
wheat spikelets infected with F. graminearum
Some gene families which were induced during Fusarium infection (differential
display RT-PCR):
Ansari et al 2007
Some protein families (examples) which were induced during Fusarium infection
(2-dimensional displays of proteins):
Zhou et al 2005
• Proteins with an antioxidant function: superoxide dismutase,
dehydroascorbate reductase, and glutathione S-transferases (GSTs)
• A 14-3-3 protein homolog was also upregulated
• PR-2 protein (β-1, 3 glucanase) was upregulated in FHB-infected spikes
Expression of proteins with highest similarity to dehydroascorbate
reductase and TaGSTF5 (a glutathione S-transferase) differed following
FHB infection in susceptible and resistant cultivars.
SOD, KAT, GR, POD and GST activity determination
wheat spikelets infected with F. graminearum
Plant material
GK Mini Manó/Nobeokabozu genetic
mapping population
(Prof. Mesterházy Ákos )
GK Mini Manó
Nobeokabozu: FHB resistant line
Arthur 71 / Sava // Rusalka / NS
171.2 /3/ F 30.74
Resistant line to e.g. leaf rust
A recombinant line from China: ‘WSY’,
Wangshubai/Sumai3/Yangangfanzhou
Gathering wheat ears and flag leaves
17. 06. 2010. Kecskéstelep,
Cereal Research Nonprofit Kht.
GK Mini Manó/Nobeokabozu wheat population
(Prof. Mesterházy Ákos )
SOD activity (U mg-1 total protein)
SOD activity
20
18
16
14
12
10
8
6
4
2
0
↓Mn resistent
↑ Mn resistent
↓ Mn sensitive
↑ Mn sensitive
KAT activity (U mg-1 total
protein)
KAT activity
40
35
30
25
20
15
10
5
0
↓Mn resistent ↑ Mn resistent ↓ Mn sensitive ↑ Mn sensitive
GR activity (U mg-1 total protein)
GR activity
1000
900
800
700
600
500
400
300
200
100
0
↓Mn resistent
↑ Mn resistent
↓ Mn sensitive
↑ Mn sensitive
Guaiacol peroxidase activity (Class III peroxidase)
POD activity (U mg-1 total protein)
POD activity
12,000
10,000
8,000
6,000
4,000
2,000
0,000
↓Mn resistent
↑ Mn resistent
↓ Mn sensitive
↑ Mn sensitive
Thank you for your attention!