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Error-Corrected DNA Synthesis
Making Genes and Genomes
Peter Carr
MIT Media Laboratory
Uses for DNA On-Demand
-minimal life
-genome rewrite
-genetic circuits
-metabolic pathways
103
individual genes
104
105
-vaccines
-gene therapy
106
Mismatch Error
Correction in Vivo
Fault-Tolerant Circuits
mismatch
“ideal”
70-mer
(MutS
bound)
Simple filter
removes 1bp
deletions
(25% -> ~0%)
70-mer
duplex
Error reduction in LacZa with
MutS/L/H filter (single cycle)
% blue colonies
(error free)
0.24
0.22
GFP gene synthesis
error rate per base: ~1 in
103 (before error
correction)
0.20
0.18
0.16
0.14
0.12
0
1
2
3
incubation length/amount
4
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