Download Diauxic Shift

Diauxic Shift
• Fermentation: energy-converting
metabolism without the involvement of an
exogenous oxidizing agent(typically
anaerobic)
• Respiration: energy-converting metabolism
with the involvement of an exogenous
oxidizing agent(typically aerobic)
Yeast Metabolism
Gene Clustering
• 16 Chromosomes
• Do genes upregulated during the diauxic
shift display chromosomal clustering?
• Or random distribution?
Expression of proteins
Expected and Control Distributions
Control Distribution
Expected Distribution
1
16
2
16
3
15
1
2
3
15
4
4
14
5
14
6
5
13
6
13
7
7
12
11
10
9
8
12
11
10
9
8
Control suggests “background” gene clustering
Control
Distribution
Expected
Distribution
2X Distribution
16
16
16
15
1 1 2 2
3 3
15
4
15
3
4
5
14
5
13
2
14
14
13
1
6
13
4
12
5
6
7
7
12 12
11 11
10
10
9
89 8
6
11
10
7
9 8
5X Distribution
Control
Distribution
Expected
Distribution
16
16 161
15
21
15
3
2
3
15
4
4
14
2
13
3
5
14
13
12
6
5
13
4
6
5
7
12
1
14
7
12
11
11
11
10
6
10
8
8
9 10 9
9X Distribution
16
15
1
2
14
3
4
13
5
12
6
11
7
10 9 8
9
8
7
Variance From Expected Gene Distribution
25
21.3 21.4
18
14.1 14.9
15
10
11.8
5.99
7.9
5.19
5
Minimum Fold Increase of Genes Sampled
10X
9X
8X
7X
6X
5X
4X
3X
2X
0
Control
Variance
20
?????????????
•
•
•
•
Variance due to a decrease in sample size?
Intrachromosomal clustering trends?
Replication of results?
Micro (clumping of a few groups of related
genes)?
• Or macro (clustering of a set of divergent
genes related through a broadly-defined
function)?