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Chapter 8 Section 3: Studying Heredity Grade 10 Biology Spring 2011 Objectives Predict the results of monohybrid genetic crosses by using Punnett squares Apply a test cross to determining the genotype of an organism with a dominant phenotype Predict the results of monohybrid genetic crosses by using probabilities Analyze a simple pedigree Punnett Square Punnettt Square: diagram that predicts the outcome of a genetic cross by considering all possible combinations of gametes in the cross Punnett Square Monohybrid Cross: ◦ Homozygous plants Punnett Square Monohybrid Cross: ◦ Heterozygous plants Punnett Square Dihybrid cross: RrYy x RrYy Test Cross Test Cross: an individual whose phenotype is dominant, but whose genotype is not known, is crossed with a homozygous recessive individual Test Cross Example: ◦ Determine the genotype of a dominant purple flower. Cross the purple flower with a homozygous recessive white flower. ◦ Dominant purple flower could have the genotypes of: PP or Pp ◦ Homozygous recessive flower has genotype of: pp Test Cross Probability Probability: the likelihood that a specific event will occur ◦ Expressed as: words, decimals, percentages, fractions Probability Number of one kind of possible outcome Total number of all possible outcomes Probability Example: if a pea plant has 2 alleles for seed colour (yellow and green), what is the probability the gamete will carry the allele for green seed colour? Yellow seed colour? ◦ Green seed colour = ½ ◦ Yellow seed colour = ½ Probability Example: Two pea plants are heterozygous for seed shape (Rr). ◦ R = dominant, round ◦ r = recessive, wrinkled ◦ Probability of each parent carrying gametes with R or r alleles = ½ ◦ Probability of offspring with RR alleles: ½x½=¼ ◦ Probability of offspring with rr alleles: ½x½=¼ ◦ Combination of Rr alleles can occur in two possible ways. One parent can contribute the R allele, and the second parent the r allele, or vice versa. ◦ Probability of offspring with Rr alleles: ¼ + ¼ = 2/4 = ½ Inheritance of Traits Pedigree: family history that shows how a trait is inherited over several generations ◦ Helpful with: genetic disorders Carriers: individuals who are heterozygous for an inherited disorder but do not show symptoms of the disorder Inheritance of Traits Pedigree: Inheritance of Traits If a gene is autosomal: it will appear in both sexes equally If a gene is sex-linked: its effects are usually seen in only males Inheritance of Traits Autosomal Dominant: every individual with the condition will have a parent with the condition Autosomal Recessive: individual with the condition can have one, two, or neither parent exhibiting the condition Inheritance of Traits Homozygous or Heterozygous Dominant: their phenotype will show the dominant allele Homozygous Recessive: their phenotype will show the recessive allele Heterozygous Carriers of Recessive Mutations: will not show mutation, can produce children who are homozygous for recessive allele if mate with another heterozygous carrier Inheritance of Traits Pedigree ◦ Be able to do evaluating a pedigree activity page 176