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Determining Probability of Genetic Outcomes Featuring the Punnett Square 8th Grade Science – K. Franklin – 8/25/10 Problem: What will the outcome be if a tall plant is crossed with a short plant? http://www.emc.maricopa.edu/faculty/farabee/biobk/biobookgenintro.html#The%20Monk%20and%20his%20peas Phenotype • Is the physical appearance of a trait. • The plant on the left has a Tall phenotype. • The plant on the right has a Short phenotype. Alleles or Genes • Are inherited “factors” (Mendel’s term) that control traits for each characteristic. • Living things have 2 for each trait. (One from each parent) • We use letters to represent alleles or genes. (t) lower case letters = recessive traits (T) capital letters = dominant traits “Tallness” is a dominant trait T = Tall “shortness” is a recessive trait t = short Genotype • Is the combination of alleles (type of genes). TT Tt tt 2 dominant alleles (purebred or “true breeder”) 1 dominant and 1 recessive (hybrid like f1) 2 recessive alleles (purebred or “true breeder”) Homozygous Dominant Heterozygous Homozygous Recessive We’ll use a Punnett Square as a graphic organizer to help us figure out the probability of outcomes. 1st: The Parent’s alleles Go on the outside Parent 2 Parent 1 1st: Write the parent’s alleles correctly. Parent 1 T Parent 2 t Purebred short t T Purebred tall 2nd: Bring the alleles from Parent 1 down. Parent 1 T T T t T T Parent 2 t T 3rd: Bring the alleles from Parent 2 over. Parent 1 T T Tt t T t Tt Parent 2 t T t 4th: Look at the different possibilities of outcomes. Parent 1 T T Tt t T t Tt Parent 2 t T t What is the Probability of a tall plant? 1. Look for the capital T 2. Count the number of squares with a dominant allele. 4 out of 4 possibilities = a 100% chance of a tall plant. T T Heterozygous t T t Heterozygous Tt The phenotype for this genotype is TALL t T t Heterozygous Tt Heterozygous What is the Probability of a short plant? 1. Look for a double recessive (tt) 2. Count the number of squares with double recessive alleles. 0 out of 4 possibilities = a 0% chance of a short plant. T Heterozygous t T t Heterozygous t T t T Heterozygous Tt Heterozygous Tt What are the possible outcomes if the hybrids are crossed? T T t t Now the first generation will serve as “parents” of the next generation. 2nd: Bring the alleles from parent 1 down. T T t t T t T t 3rd: Bring the alleles from Parent 2 over to the right. T t T T T T t t T t t t 4th: Check the probability T Homozygous Dominant T T T t Heterozygous T t 1 out of 4=25% Heterozygous t T t Homozygous Recessive t t 1 out of 4=25% What is the probability of a short Phenotype? T Homozygous Dominant T T T Tall Heterozygous t t Heterozygous T t Tall Homozygous Recessive T t t t Tall Short 1 out of 4=25% What is the probability of a Homozygous Recessive Genotype? T Homozygous Dominant T T T Heterozygous t T t t Heterozygous T t Homozygous Recessive t t 1 out of 4=25% More Practice • Read all about it! Textbook pp. 62-64 • Use one of these websites for additional practice as a class or for enrichment at home: – http://www2.edc.org/weblabs/Punnett/punnettsqua res.html – http://www2.edc.org/weblabs/Mendel/MendelMenu .html Now You Try! Complete the worksheet that your teacher gave you!