Download P1_050506

Thanking you for the attention
Participation in SB – P1 (NIO)
• WP 0 – Project Managements
• WP 1 – Mechanisms for establishment of dormant stage
• WP 2 – Mechanisms for arousal from dormant stages
• WP 4 – Engineering/Genetic testing strategies
• WP 5 – Discovery of common and divergent pathways for dormant
stages, dormancy and desiccation resistance
• WP 6 – Dissemination of results
Workpackage 1 (WP1): Mechanisms for
establishment of dormant stage
Deliverables
Deliverable
Number
Deliverable
date
Optimal conditions for inducing
asexually and sexually reproducing
rotifers and their resting eggs
D4
M9
cDNA libraries, EST sequencing
D5
M16
Pattern of global gene expression
during formation of resting eggs by
miroarray analysis
D6
M27
Quantitative analyses of the expression
of specific genes by QPCR
D7
M35
Proteins and metabolites identified to
be associated with formation of resting
eggs or contained within them
D8
M36
Workpackage 2 (WP2): Mechanisms for
arousal from dormant stages
Deliverables
Deliverable
Number
Deliverable
date
Patterns of global gene expression
during exit from dormancy of rotifer
resting eggs by microarray analyses
D11
M35
Quantitative expression levels of
specific genes during resumed
development by QPCR
D12
M35
Proteins and metabolites associated
with resumed metabolic activities
during embryonic development in
restings eggs and asexual rotifer
resting eggs
D13
M30
Workpackage 4 (WP4):Engineering/Genetic
testing strategies
Deliverables
Deliverable
Number
Deliverable
date
Differences in the transcriptome,
proteome and metabolome analyses
between asexual and sexual rotifer
strains
D20
M35
Workpackage 5 (WP5):Discovery of common
and divergent pathways for dormant stages,
dormancy and desiccation resistance
Deliverables
Deliverable
Number
Deliverable
date
Construction of the databases
D24
M30
Identification of species- specific and
conserved genes, proteins and
compounds
D25
M33
Development of in silco models for cell
dormancy or desiccation tolerance
D26
M36
Production of a comprehensive report
D7
M35
Workpackage 6 (WP6):Dissemination of
results
Deliverables
Deliverable
Number
Deliverable
date
Participation in scientific meetings
D28
M36
A workshop on dormancy:
opportunities and challenges:
preservation of cells and organisms
D29
M24*
Publication of a book on dormancy
D30
M36*
* To
be decided at the meeting
P1- NIO: Goals for M6
Goals for M12-16
WP1: Tasks
Deliverable
Number
Asexual and sexually reproducing
rotifer cultures
D4
M9
Construction of cDNA libraries (P5):
a) Asexually reproducing rotifers
b) Sexually reproducing rotifers
c) Subtractive library ??
EST’s (P5): ~ 5,000 clones per library
(~15,000 ESTs for rotifers)
D5
M16
P1- NIO: Goals for M12-16
WP2: Tasks
Deliverable
Number
Collection of asexual rotifer eggs and
resting eggs samples for proteome
profiling
D4
WP5: Tasks
Deliverable
Number
Compile information on gene and cDNA
sequences of rotifers for data base
D24
M9
M30