Download Serologi Forensik

Farmasi Forensik
Mayagustina Andarini
Sarjana
Apoteker
M.Sc
: Fakultas Farmasi UGM
: Fakultas Farmasi UGM
: Div.Molecular Toxicology, Department of
Pharmacochemistry, Faculty of Science, Vrije Universiteit
Amsterdam
Pekerjaan :
 Dosen tidak tetap FF-UPS
 Direktorat Penilaian OT, SM dan Kosmetik,
Badan POM RI
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Pendahuluan
Darah
Golongan Darah
Sistem Faktor Rhesus
Transfusi Darah
Penyidikan Darah dengan Polimorfisme
Pewarnaan Darah
Pemeriksaan Darah untuk Kasus Kriminal
Serology is the scientific study of blood serum.
In practice, the term usually refers to the diagnostic
identification of antibodies in the serum.
Such antibodies are typically formed in response to :
1. an infection (against a given
microorganism),
2. against other foreign proteins (in
response, for example, to a mismatched
blood transfusion),
3. or to one's own proteins (in instances of
autoimmune disease)
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Forensic science
a multidisciplinary subject used for examining crime scenes and gathering
evidence to be used in prosecution of offenders in a court of law.
Forensic science techniques are also used to examine compliance with
international agreements regarding weapons of mass destruction.
The main areas used in forensic science are biology, chemistry, and medicine,
although the science also includes the use of physics, computer science,
geology, and psychology.
Forensic scientists examine objects, substances (including blood or drug
samples), chemicals (paints, explosives, toxins), tissue traces (hair, skin), or
impressions (fingerprints or tidemarks) left at the crime scene.
The majority of forensic scientists specialize in one area of science.
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Subdivisions
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Computational forensics concerns the development of algorithms and software to assist forensic examination.
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Criminalistics is the application of various sciences to answer questions relating to examination and comparison of biological evidence, trace evidence, impression evidence
(such as fingerprints, footwear impressions, and tire tracks), controlled substances, ballistics, firearm and toolmark examination, and other evidence in criminal investigations.
Typically, evidence is processed in a crime lab.
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Digital forensics is the application of proven scientific methods and techniques in order to recover data from electronic / digital media. Digital Forensic specialists work in the
field as well as in the lab.
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Forensic anthropology is the application of physical anthropology in a legal setting, usually for the recovery and identification of skeletonized human remains.
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Forensic archaeology is the application of a combination of archaeological techniques and forensic science, typically in law enforcement.
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Forensic DNA analysis takes advantage of the uniqueness of an individual's DNA to answer forensic questions such as paternity/maternity testing or placing a suspect at a crime
scene, e.g. in a rape investigation.
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Forensic entomology deals with the examination of insects in, on, and around human remains to assist in determination of time or location of death. It is also possible to
determine if the body was moved after death.
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Forensic geology deals with trace evidence in the form of soils, minerals and petroleums.
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Forensic meteorology is a site specific analysis of past weather conditions for a point of loss.
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Forensic odontology is the study of the uniqueness of dentition better known as the study of teeth.
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Forensic pathology is a field in which the principles of medicine and pathology are applied to determine a cause of death or injury in the context of a legal inquiry.
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Forensic psychology is the study of the mind of an individual, using forensic methods. Usually it determines the circumstances behind a criminal's behavior.
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Forensic seismology is the study of techniques to distinguish the seismic signals generated by underground nuclear explosions from those generated by earthquakes.
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Forensic toxicology is the study of the effect of drugs and poisons on/in the human body.
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Forensic document examination or questioned document examination answers questions about a disputed document using a variety of scientific processes and methods. Many
examinations involve a comparison of the questioned document, or components of the document, to a set of known standards. The most common type of examination involves
handwriting wherein the examiner tries to address concerns about potential authorship.
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Forensic video analysis is the scientific examination, comparison, and evaluation of video in legal matters.
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Forensic engineering is the scientific examination and analysis of structures and products relating to their failure or cause of damage.
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Forensic limnology is the analysis of evidence collected from crime scenes in or around fresh water sources. Examination of biological organisms, particularly diatoms, can be
useful in connecting suspects with victims.
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Forensic Accounting is the study and interpretation of accounting evidence
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Forensic Botany is the study of plant life in order to gain information regarding possible crimes
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Forensic Dactyloscopyis the study of fingerprints(patent and latent fingerprints)
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Trace Evidence Analysis is the analysis and comparison of trace evidence including(glass, paint, fibers, hair, etc.
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Forensic Chemistry is the study of detection and identification of illicit drugs, accelerants used in arson cases, explosive and gunshot residues
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Forensic Optometry is the study of glasses and other eye wear relating to crime scenes and criminal investigations
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Forensic Serology is the study of blood groups and blood for identification purposes following a crime,the study of how blood splaters, and the analysis of blood stains
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Forensic Linguistics deals with anything in the legal system that requires linguistic expertise
Serologi Forensik:
Untuk menentukan tipe dan karakterisasi
darah, tes darah, uji pewarnaan darah, dan
penyiapan tanda bukti.
Analisis semen, saliva atau cairan tubuh
lainnya, baik yang melibatkan tipe DNA atau
tidak.
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Penting sebagai bukti kriminal
Menentukan posisi dan tindak pidana
Blood is the most common, well-known, and perhaps most important
evidence in the world of criminal justice today.
There's no substitute for it, whether for medical or forensic purposes.
Its presence always links suspect and victim to one another and the scene
of violence.
Bloodstain patterns tell a lot about position and movement during the
crime, who struck whom first, in what manner, and how many times.
This destroys most alibi and self-defense arguments for crime, and at the
very least, trips most suspects up in their explanation of what happened.
Over the years, criminals have tried many ingenious ways to hide, clean
up, and remove blood evidence, but it's an area where criminal justice
technology has always stayed one step ahead of them.
Slightly Alkaline fluid: water, cells, enzymes, proteins, and inorganic substances
that circulate throughout the vascular system carrying nourishment and
transporting oxygen and waste.
The most fluid portion  plasma, (water, and serum)
yellowish and contains white cells and platelets.
The most non-fluid portion  red cells
Medical scientists  white cells
Forensic scientists  red cells and secondly with serum.
Serum  determine the freshness of a blood sample because serum clots several
minutes after exposure to air (a centrifuge is necessary to separate clotted
material from the rest of serum).
In serum are also found antibodies, which have important forensic implications.
With red cells, the analyst looks for smaller substances residing on their surfaces,
such as antigens, which have important forensic implications.
One might even say that forensic serology is all about antigens and antibodies,
but that is the domain of immunology.
Pada hukum forensik, darah sbg kekuatan barang bukti:
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Tipe golongan darah individu
Memperkirakan hubungan orang tertentu dg orang lain
Profil antibodi berbeda walaupun pd kembar identik
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Tipe Gol.darah: Sistem A-B-O, th 1901 LANDSTEINER
Th 1937: Reaksi antigen-antibodi  ABH, Mn, Rh & Gm
(diantara >100 antigen yang ada)
Rh (faktor Rhesus) lbh dikenal  D-antigen
Antigen: struktur kimia yg melekat
pd permukaan sel darah merah
Antibodi: protein yang mengambang pada cairan
darah
Prinsip dasar serologi:
Setiap ada antigen, akan terbentuk antibodi yang spesifik
Golongan Darah :
Antigen pada sel darah merahnya dan ada antibodi terhadap antigen tersebut
di dalam serumnya.
A blood type (also called a blood group) is a classification of blood based on
the presence or absence of inherited antigenic substances on the surface of
red blood cells (RBCs). These antigens may be proteins, carbohydrates,
glycoproteins, or glycolipids, depending on the blood group system. Some
of these antigens are also present on the surface of other types of cells of
various tissues. Several of these red blood cell surface antigens that stem
from one allele (or very closely linked genes), collectively form a blood
group system.
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BLOOD TYPE IS GENETIC
The A and B antigen molecules on the surface of red blood cells are
produced by two different enzymes. These two enzymes are encoded
by different versions, or alleles, of the same gene: A and B.
The A and B alleles code for enzymes that produce the type A and B
antigens respectively. A third version of this gene, the O allele, codes
for a protein that is not functional and does not produce surface
molecules. Two copies of the gene are inherited, one from each parent.
The possible combinations of alleles produce blood types in the
following way:
Blood Types and the Population
O positive is the most common blood type. Not all ethnic groups have the same mix of
these blood types.
Hispanic people, for example, have a relatively high number of O’s, while Asian
people have a relatively high number of B’s.
Rh Blood Types
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Rh blood types were discovered in 1940 by Karl Landsteiner and Alexander Wiener. This was 40 years
after Landsteiner had discovered the ABO blood groups. Over the last half century, we have learned far
more about the processes responsible for Rh types. This blood group may be the most complex genetically
of all blood type systems since it involves 45 different antigens on the surface of red cells that are
controlled by 2 closely linked genes on chromosome 1.
The Rh system was named after rhesus monkeys, since they were initially used in the research to make
the antiserum for typing blood samples. If the antiserum agglutinates your red cells, you are Rh+ . If it
doesn't, you are Rh- . Despite its actual genetic complexity, the inheritance of this trait usually can be
predicted by a simple conceptual model in which there are two alleles, D and d. Individuals who are
homozygous dominant (DD) or heterozygous (Dd) are Rh+. Those who are homozygous recessive (dd) are
Rh- (i.e., they do not have the key Rh antigens).
Clinically, the Rh factor, like ABO factors, can lead to serious medical complications. The greatest problem
with the Rh group is not so much incompatibilities following transfusions (though they can occur) as those
between a mother and her developing fetus. Mother-fetus incompatibility occurs when the mother is Rh(dd) and the father is Rh+ (DD or Dd). Maternal antibodies can cross the placenta and destroy fetal red
blood cells. The risk increases with each pregnancy. Europeans are the most likely to have this problem-13% of their newborn babies are at risk. Actually only about ½ of these babies (6% of all European births)
have complications. With preventive treatment, this number can be cut down even further. Less than 1%
of those treated have trouble. However, Rh blood type incompatibility is still the leading cause of
potentially fatal blood related problems of the newborn. In the United States, 1 out of 1000 babies are born
with this condition.
Rh type mother-fetus incompatibility occurs only when an Rh+ man fathers a child with an Rhmother. Since an Rh+ father can have either a DD or Dd genotype, there are 2 mating combinations
possible:
Only the Rh+ children (Dd) are likely to have
medical complications. When both the mother and
her fetus are Rh- (dd), the birth will be normal.
Human fetus in a mother's
uterus (the umbilical cord and
placenta connect the fetus to its
mother)
Rh antibodies are harmless until the mother's second or later pregnancies. If she is ever carrying another Rhpositive child, her Rh antibodies will recognize the Rh proteins on the surface of the baby's blood cells as
foreign, and pass into the baby's bloodstream and attack those cells. This can lead to swelling and rupture of the
baby's RBCs. A baby's blood count can get dangerously low when this condition, known as hemolytic or Rh
disease of the newborn, occurs
If a father's Rh factor
genes are + +, and the
mother's are + +, the
baby will have one +
from the father and
one + gene from the
mother. The baby will
be + + Rh positive.
If a father's Rh factor
genes are + +, and
the mother's are - -,
the baby will have
one + from the father
and one - gene from
the mother. The baby
will be + - Rh
positive.
If the father's
genes are - -, and
the mother's are -, the baby will
be: - - Rh
negative
If the father's genes
are + - Rh positive,
and the mother's are +
- Rh positive, the
baby can be:
+ + Rh positive
+ - Rh positive
- - Rh negative
If the father's genes are -, and the mother's are +
-, the baby can be:
+ - Rh positive
- - Rh negative
Why is Rh disease a concern?
When an Rh negative mother has a baby that is Rh positive, problems can develop if the baby's red blood cells cross to the Rh
negative mother. This usually happens at delivery when the placenta detaches. It may also happen, however, anytime blood
cells of the two circulations mix such as during a miscarriage or abortion, with a fall, or during an invasive prenatal testing
procedure such as an amniocentesis or chorionic villus sampling.
The mother's immune system sees the baby's Rh positive red blood cells as foreign. Just as when bacteria invade the body, the
immune system responds by developing antibodies to fight and destroy these foreign cells. The mother's immune system
keeps the antibodies in case the foreign cells appear again, even in a future pregnancy. The mother is now Rh sensitized.
Although it is not as common, a similar problem of incompatibility may happen between the blood types (A, B, O, AB) of the
mother and baby in the following situations:
Mother's Blood Type O A B Baby's Blood Type A or B B A In a first pregnancy, Rh sensitization is not likely. Usually it only
becomes a problem in a future pregnancy with another Rh positive baby. During that pregnancy, the mother's antibodies
cross the placenta to fight the Rh positive cells in the baby's body. As the antibodies destroy the red blood cells, the baby can
become anemic. The anemia can lead to other complications including jaundice and organ enlargement.
Rh disease is also called erythroblastosis fetalis during pregnancy. In the newborn, the resulting condition is called hemolytic
disease of the newborn (HDN).
Some of the more common complications of Rh disease for the fetus and newborn baby include the following:
anemia (in some cases, the anemia is severe with enlargement of the liver and spleen)
jaundice - yellowing of the skin, eyes, and mucous membranes.
severe anemia with enlargement of the liver and spleen
hydrops fetalis - this occurs as the fetal organs are unable to handle the anemia. The heart begins to fail and large amounts of
fluid build up in the fetal tissues and organs. A fetus with hydrops fetalis is at great risk of being stillborn.
After birth, the red blood cell destruction may continue. Problems may include the following:
severe jaundice
The baby's liver is unable to handle the large amount of a substance called bilirubin that results from red blood cell.
breakdown. The baby's liver is enlarged and anemia continues.
kernicterus
The most severe form of too much bilirubin and results from the build up of bilirubin in the brain. This can cause seizures,
brain damage, deafness, and death
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Population data
The frequency of Rh factor blood types and the RhD neg allele gene differs in various
populations.
Population data for the Rh D factor and the RhD neg allele[