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Dihybrid Punnett Squares What exactly is a dihybrid cross? Predicting the outcomes from crossing 2 traits. Why do we use them? Instead of doing to monohybrid crosses, you can combine them to see all the possible combinations from that particular mother and father. Dihybrid Cross Traits: Seed shape & Seed color Alleles: R round r wrinkled Y yellow y green RrYy RY Ry rY ry x RrYy RY Ry rY ry All possible gamete combinations Dihybrid Cross RY RY Ry rY ry Ry rY ry Dihybrid Cross RY Ry rY RY RRYY RRYy RrYY RrYy Ry RRYy RRyy RrYy Rryy rY RrYY ry RrYy RrYy Rryy rrYY rrYy ry rrYy rryy Round/Yellow: Round/green: 9 3 wrinkled/Yellow: 3 wrinkled/green: 1 9:3:3:1 phenotypic ratio Incomplete Dominance When the heterozygous condition is intermediate between dominant and recessive Happens because one allele is defective, example: R=red flowers, R’= white flowers so RR’ = pink flowers (R codes for enzyme to produce red pigment, R’=no enzyme) RR x R’R’ Incomplete dominance R’R’ x R’R’ Red White F1 generation: 1 Red: 2 Pink: 1 white Codominance Heterozygote expresses both alleles’ conditions Ex. A black rooster bred with a white hen produces a black and white checkered chicken. Usually uses both capital letters (black= B, white=W , checkered = BW) Codominance practice: cross a black rooster with a white hen Key: W W B = black W = white B BW BW BW = checkered Results: 100% checkered (BW) B BW BW Now let’s cross a checkered hen with a checkered rooster. B W B BB BW W BW WW Results: 25% Black 50% Checkered 25% White (1:2:1) Codominance Two alleles are expressed (multiple alleles) in heterozygous individuals. Example: blood type 1. 2. 3. 4. type type type type A B AB O = = = = IAIA or IAi IBIB or IBi IAIB ii Blood type quick facts Red blood cells are called erythrocytes Proteins on their surfaces are called antigens, controlled by genes Antigens make antibodies to foreign substances, which includes RBCs with different antigens on their surface 4 phenotypes: A, B, AB, O 3 alleles: IA, IB, i Phenotype A Surface molecule A • The lA allele is dominant to i, so inheriting either the lAi alleles or the lA lA alleles from both parents will give you type A blood. • Surface molecule A is produced. Phenotype B • The lB allele is also dominant to i. • To have type B blood, you must inherit the lB allele from one parent and either another lB allele or the i allele from the other. • Surface molecule B is produced. Surface molecule B Phenotype AB • The lA and lB alleles are codominant. • If you inherit the lA allele from one parent and the lB allele from the other, your red blood cells will produce both surface molecules and you will have type AB blood. Surface molecule B Surface molecule A Phenotype O •No antigens produced Blood Group Antigens Antibodies Can receive from Can give to A A B A or O B B A B or O AB A and B None O None both A, B, AB, O O A or AB B or AB AB A, B, AB, O Codominance Problem Example:homozygous male Type B (IBIB) x heterozygous female Type A (IAi) IA i IB IAIB IBi IB IAIB IBi 1/2 = IAIB 1/2 = IBi Another Codominance Problem Example: male Type O (ii) x female type AB (IAIB) IA IB i IAi IBi i IAi IBi 1/2 = IAi 1/2 = IBi