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Transcript 
The Cell Cycle
Overview

What are the various stages of the cell cycle?
DNA Replication

What is meant by
semiconservative
replication and how
did Meselson and Stahl
demonstrate that is is
what took place?
DNA Replication

What is theta replication
and where does it take
place?
DNA Replication

What are replicons?

What is an origin
recognition complex?

What is an
autonomously
replicating sequence?
DNA Replication

What components are needed for DNA
replication?

What is the function of DNA polymerase?

In which directions does DNA synthesis
occur?
DNA Replication
DNA Replication

What are Okazaki
fragments?

What is the function of
DNA ligase?
DNA Replication

How are mistakes in DNA corrected?
DNA Replication

What role does RNA
play in DNA synthesis?

What is primase?
DNA Replication

What is involved in unwinding the double helix in
preparation for replication?
DNA Replication
DNA Replication
DNA Replication
DNA Replication

What is meant by the
end-replication
problem?
DNA Replication

How do eukaryotic cells
handle the endreplication problem?

What is meant by DNA
licensing?
DNA Damage and Repair

What kinds of
spontaneous mutations
regularly alter DNA
molecules?

What kind of mutagens
alter DNA molecules?
DNA Damage and Repair

What are the different
types of repair
mechanisms used to
correct alterations in
DNA?
Nuclear and Cell Division
Nuclear and Cell Division
Nuclear and Cell Division
Nuclear and Cell Division

What is the difference between kinechore and polar
microtubules?
Nuclear and Cell Division

What influences the length of spindle fibers?
Nuclear and Cell Division

What is responsible for the alignment of
chromosomes during metaphase?

What is involved in the splitting of the
chromatids?
Nuclear and Cell Division

What kinds of motor
proteins are involved in
chromosome
movement?
Nuclear and Cell Division

How does cytokinesis differ in animals and
plants?
Regulation of Cell Cycle

What are the cell cycle checkpoints?
Regulation of Cell Cycle

What is MPF and how do we know it exists?
Regulation of Cell Cycle

How were mutant yeast cells used to find the
molecule that controlled the cell cycle?

What kind of protein is produced by the cdc 2
gene?

What are cyclins?
Regulation of Cell Cycle

What are mitotic cyclins?
Regulation of Cell Cycle

Besides the availability of cyclins, what else controls
the activity of MPF?
Regulation of Cell Cycle

How does the active
MPF bring about events
of mitosis intiation?
Regulation of Cell Cycle

How is G1 checkpoint controlled?
Regulation of Cell Cycle

How does anaphase
promoting complex
control spindle
assembly checkpoint?

What ensures the proper
timing of these events?
Regulation of Cell Cycle
Cell Growth and Cancer

How is the ras pathway involved in the cell cycle
control?
Cell Growth and Cancer

How can growth factors inhibit cell division?

Alterations in what kinds of genes lead to
development of cancer?

How do oncogenes cause cancer?
Cell Growth and Cancer

How does the p53
tumor suppressor gene
work?
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