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How Genes Work
13.1 Gene function
What are genes and what do they do?

Section of DNA which codes for a polypeptide

Genotype determines phenotype
What is the central dogma?

Explains process of polypeptide manufacture
Genetic code is
“read” here
transcription
DNA
(as a gene)
synthesis
translation
mRNA
Polypeptide chain
(folds into a
___________)
What types of RNA are used?



Recall DNA vs. RNA
tRNA
rRNA


Found in ribosomes along with ______
mRNA
What is the genetic code?


Triplets or codons
Template strand
Do all organisms use the same code?

Yup!

Some minor deviations,
but…
Why not just use DNA to make a protein?
How Genes Work
13.2 Transcription
What is transcription?



First step in polypeptide production
Occurs in ___________
Players






DNA coding strand
RNA polymerase
Nucleotides
ATP
Promotor region
Final product: immature mRNA

In prokaryotes, it is mature at this point
What are the three steps to transcription?

Initiation



Binding at promotor
Elongation
Termination

Stop sequence
How does mRNA become mature?

Post-transcriptional modifications




Note: this is only in eukaryotes
5’ cap
3’ poly-A tail
Introns removed

Exons spliced together
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