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How Genes Work 13.1 Gene function What are genes and what do they do? Section of DNA which codes for a polypeptide Genotype determines phenotype What is the central dogma? Explains process of polypeptide manufacture Genetic code is “read” here transcription DNA (as a gene) synthesis translation mRNA Polypeptide chain (folds into a ___________) What types of RNA are used? Recall DNA vs. RNA tRNA rRNA Found in ribosomes along with ______ mRNA What is the genetic code? Triplets or codons Template strand Do all organisms use the same code? Yup! Some minor deviations, but… Why not just use DNA to make a protein? How Genes Work 13.2 Transcription What is transcription? First step in polypeptide production Occurs in ___________ Players DNA coding strand RNA polymerase Nucleotides ATP Promotor region Final product: immature mRNA In prokaryotes, it is mature at this point What are the three steps to transcription? Initiation Binding at promotor Elongation Termination Stop sequence How does mRNA become mature? Post-transcriptional modifications Note: this is only in eukaryotes 5’ cap 3’ poly-A tail Introns removed Exons spliced together