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Molecular Biology
Lecture 23
Chapter 20
DNA repair
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Fig. 20.29
Fig. 20.31
Fig. 20.28
Fig. 20.32
Excision repair
Base excision repair:
Most prevalent and works
on subtle changes in DNA
structure caused by chemical
modifications
Nucleotide excision repair: Deals with more drastic
changes to bases causing
distortion of the DNA double
helix
Fig. 20.33
Base excision repair
Fig. 20.36
Nucleotide excision repair
12-13 base long
Fig. 20.39
Mismatch repair
Fig. 20.40
Recombination
repair
Fig. 20.41
Error prone (SOS) bypass
List of problems to review before the final examination
Recombinant DNA technology
Southern blot
RT-PCR
Plasmids and host strains
PCR for cloning
Site-directed mutagenesis
Restriction mapping
Gene regulation in prokaryotes
LAC operon
TRP operon
R-loop hybridization experiments
Gene regulation in eukaryotes
Regulation of GAL genes in yeast
Post-transcriptional control (Tr receptor and ferritin)
Chromatin structure and gene expression (figure 13.21 and 13.22)
DNA replication
Mechanism and proteins participating in DNA replication