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Transcript 
Last class
Cloning
Transformation
Paper 2 intro
Plasmid prep
Plasmid in cells
How to get out?
Plasmid prep
Plasmid prep: 2 Precipitate Proteins & DNA
Plasmid prep: 3 Bind plasmid
Plasmid prep: 3 Bind plasmid
Plasmid prep: 3 Bind plasmid
Plasmid prep: 4 Elute plasmid
Plasmid prep & RE mapping
Lyse bacteria, isolate plasmid pBK-CMV with gene
RE digest and run on a gel
Determine RE map of plasmid (size of insert = 5.5Kb)
Implied knowledge!
What were some things you had to look up?
First experiments
Purpose?
Experiment?
Results?
First experiments
Purpose?
Experiment?
Results?
Next step?
c-Myc?
Purpose?
Experiment?
Results?
Control: Prediction for iPSC expression pattern
Purpose?
Experiment?
Prediction for miR iPSCs?
Control: Prediction for exogenous genes?
Purpose?
Experiment?
Prediction for miR iPSCs?
What else?
miRNA iPSCs?
Purpose?
Experiment?
Prediction for miR iPSCs?
ChIP
Y
Y
Y
Y
Y
Unbind & sequence
Y
ChIPs?
Purpose?
Experiment?
Result?
ChiPs + Expression = ?
% GFP +ve colonies = ?
Purpose?
Experiment?
Results?
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