Download Rough Sheath 2

rough sheath2 (rs2)
By Charlene Kormondy and
Sarah Higbee-Tindell
rough sheath2 (rs2), CMK, SRH, 1-13-05
Table of Contents
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Information
What’s wrong with the mutant
DNA sequence
DNA sequence
explanation
• References
Information
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Bass lab family number: 1558
The rs2 gene has been cloned
The gene is on chromosome 1
The mode of inheritance is recessive
What is wrong with the mutant?
The rs2 mutant has warty distorted leaf blade & sheath.
Normal
Plant
Mutant
Plant
DNA sequence of rs2
Zea mays rough sheath2 (rs2) cDNA gene sequence
>gi|4583430|gb|AF126489.1|AF126489
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
CGCGCATTAT
CAGCGCTGGC
CGCGAGTGGC
TGCCTGGAGC
GAGGAGCAGC
GCCGCCGAGG
GAGAAGCAGC
GAGCGGGGCA
CCACAGCAGG
TGGCTGTCGT
CCCAGACCGC
CCGCCCGCGC
TTCCCGAGCC
CAGGCGCTGG
GCGGCGCACC
GAGCGCGAGA
CGGCTGGAGC
GAGCTCCGCC
CACGCGCGCG
GCCACCGACA
TCGCGTGCTA
CCATGGATTC
AACTGTTGTT
AAGAAATGTA
AAGGTGTGGT
GGCCTGAGGA
ACCTGGTGTC
GCTGGAAGAA
GCCTGGTGAT
TGCCCGGGCG
AGCGGGAGCT
GGTACGAGTG
CTGCCGCTGC
CCAACGCCGG
CGTCGCCGTC
CCGCGCCATG
CGTCGCAGCA
CGGAGCTCGC
GGCGGGAGGC
TGCGGCGGCG
AGGCGGCCGC
GCGACGCGCA
TCGCCAAGTT
TGAACTGTTG
CGTGCTCCGG
CCTTGCTCAC
ACGGTCGGTT
ATGCTGCCGT
GGTGAGGGAT
AGACGCCGTC
GCAGCGCATG
CTACCTCCGC
CCGCCTGCAG
CACGGCCAAG
GAGGGACAGC
GCTGCTCGAG
GCCGTCGCCG
GCCGGCCGCC
CGTGACGCTC
GATGCCGGAT
CGGCGGCGCC
CGAGTGCTGC
GGCGTGGCGG
CGAGGTGTGG
CGCGGAGCGC
GGTCAAGGAG
CGTCGAGCAG
AACTGGACAA
TTCACGGCTT
GTTCACTTCC
CAATGCTTCT
TACTGTCGTA
GGGCGATGGC
CTGCGCGCCT
AACGTGGCCC
CCCGGGATCA
GCCAAGCACG
CGCCTCGGCA
CGCCGCCCGC
AACTTCGCGG
CTGCTCATGG
GCCGCCGCCG
AGCCTCGCGT
CGCGCCGCGG
GCGCCCCCGG
CGCGAGCTGG
CTGAAGCGCG
GAGGAGTTCG
GTGGAGCGGG
GAGAAGATGG
ATGGGGGGGT
ACGAGCCGCT
TCTTGCATTC
GCTTTCTTTT
CTCTCTATCT
GTTTCATCCT
CTTTGCAGAT
ACGTCCGGCA
TCGACCGCGA
AGAAGGGCTC
GCAACAAGTG
AGTGGTGGGA
CGCCGGAGCC
AGAAGCTCGT
CCGCGCCCGT
CCGCCGTCGC
CCGCCGCGGT
CCGACGCGGC
GCATGGCGGT
AGGAGGGGCG
TGGAGCAGCA
AGGCCAAGAT
ACCACCGGGA
CCGAGCAGTG
GCTCCCGTTC
GACCACACCG
TGGGACGCGA
TTTCTTTTCT
ACTAGTAGTT
GAAGGAGCGA
GTACGGCCCC
CGCCAAGTCG
GCTCACCGAG
GAAGAAGATC
GGTGTTCAAG
TAGCCCCGAC
CGGAGAGAGG
GCTGCCGCCC
GCACCCGCCG
GGCGCCGGGC
GCCGTACGGG
CGTGGACGGG
GCGCGCGTGG
GCTGGAGATG
GCGCACCATG
GAAGGTGGCC
GGCCGCCAAG
ATGGTCCTCT
TCGCTAGCTA
CTTGTAAACG
TTTTATTTAC
GATCAGAGTC
DNA sequence Explanation
DNA = DeoxyriboNucleic Acid
A = Adenine
G = Guanine
C = Cytosine
T = Thymine
A & T go together
G & C go together
First strand = GCTA
Second strand =CGAT
Reference
Hadjinov, M.I. (1937)
Genes of “Rough Sheath” in maize.
Bull Appl Bot Gen Plant Breed Ser II 7:247-258.
Thanks to:
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Dr. Hank Bass
Ms. Debbie Figueroa, graduate student
Ms. Bobbye Hill, biologist
Dr. Brian Ring, post-doctoral research
associate
• And of course, the wonderful Mrs. Crow
Field placard
Gene Name:
rough sheath2, rs2
Locus:
Chromosome # 1
Placard By:
Charlene Kormondy &
Sarah Higbee-Tindell
The School of Arts and Sciences
Middle School Mentorship
THE END