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Genomics for Triticeae improvement
FP7 European Project
Overview
Achieve significant progress in Triticeae genomics and support efficient
breeding of improved varieties for European agriculture
 European Seventh Framework Programme project (financed by the EC)
 Project duration: 4 years – From June 2008 to Mai 2012
 Budget: 7,5 million Euro (5,3 million Euro supported by the EC)
 Project Coordinator: Catherine Feuillet (INRA)
 Activities: 5 research WPs, 1 WP for project dissemination and 1 WP for
project management
Overview
 17 partners (including 2 breeders) from 9 countries
Public
Private
Activities and WPs
WP1: Physical mapping
Of wheat group 1 and 3 chromosomes

Construction of BAC libraries

Fingerprinting of BAC libraries

Assembly of BAC contigs

BAC libraries pooling from the MTP and rearraying for
BES (by INRA-CNRGV)

BAC end sequencing of the MTPs for marker
development and genome analysis
WP1: Physical mapping

Assembly of BAC contigs
•
Chromosome 3B: assembly by INRA (France)
•
Chromosome 3D: assembly of 3DS by IEB (Czech Republic),
and assembly of 3DL by JIC (UK)
•
Chromosome 1B: assembly of 1BS by HU (Israel), and assembly
of 1BL by INRA (France)
•
Chromosome 1A: assembly of 1AS by UZH (Switzerland), and
assembly of 1AL by SU (Turkey)
WP2: Physical maps anchoring
Of wheat and barley group 1 and 3 chromosomes

Establishment of a marker repository: including only markers for
which sequences are available

Generating resources for high resolution genetic mapping: For
wheat, creation of a population of CS x Re RILs of about 2000
plants. For barley, creation of Morex x Barke RILs.

Anchoring BAC contigs to chromosomes by high throughput
screening methods: forward and reverse anchoring

Comparative genetic and physical mapping with other grass
genomes
WP3: Gene isolation and new alleles discovery
Isolate five agronomically important genes and QTLs (4 in wheat and 1
in barley)
Trait
Species
Chr.
Partner
Stripe rust resistance YrH52
Wild emmer wheat 1BS
HU
Wheat quality (pentosan
viscosity) PV-QTL
Bread wheat
1BL
INRA
Glume blotch resistance
QSng.sfr–3BS
Bread wheat
3BS
UZH
Grain yield QYld.idw-3BS
Durum wheat
3BS
UNIBO
Plant architecture (tillering)
cul4
Barley
3H
UMIL
WP3: Gene isolation and new alleles discovery

High-resolution mapping of the genes underlying the
target traits

Identification of candidate genes

Functional validation of candidate genes

New alleles discovery
WP4: Molecular markers for breeding
WP leading by the breeding companies

Development of trait specific markers: using markers from
WP1 (BAC end sequencing) and WP2

Establishment of an European diversity panel for
association mapping: panel contains 730 varieties (171 from
UK, 178 from Germany and 381 from France)
The data generated will be useful for all the chromosomes.
WP5: Bioinformatics tools and databases
For wheat and barley

Development of a web interface integrating genetics and physical
maps

Development of a new analytical framework for contig assembly:
LTC (more robust than FPC)

Development of comparative genome informatics tools: with
Brachypodium, rice, sorghum, etc…

Development of a new version of TriAnnot pipeline for automated
sequence annotation
WP6: Dissemination, technology transfer and training

Organisation of 9 training courses in the different
partners instituts

Development of the Project website
http://www.triticeaegenome.eu/

Dissemination at scientific and technological events:
talks/posters, production of a project leaflet and a poster
WP7: Project Management

Scientific and technical management

Administrative and financial management: organization of
the different project meetings, monitoring of the project costs

Communication and interactions between partners and
other projects: establishment of a Collaborative Platform,
interaction with the Triticeae Genomics Network (TGN)

Technical and financial reporting to the EC: detailed
reports that describe the work progress, costs incurred and other
justifications for the reimbursement of project costs
A large network of international collaborators
UMB
Iowa
SU
MPI Cologne
Minnesota
SU
CSIC
CAAS
Beijing
Modena U
Okayama U
CRA-CER
Foggia
UC Davis
Viterbo U
Kansas
SU
UC
Riverside
CIMMYT
CSIRO
Murdoch U
INTA
ACPFG