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Myocardial Fatty Acid Metabolism
Fatty acid: albumin
LPL
VLDL-TAG
Legend
Chylomicron-TAG
Cell
FATP
CD36
brane
mem
MLYCD
AMP+PP
P PPi
ACAC
Acyl-CoA
Malonyl CoA
Fatty acyl CoA
F
Acylcarnitine
Carnitine
CPT1
Acylcarnitine
Carnitine
3-Hydroxyacyl-CoA
yac
c
cyl-CoA
dehydrogenase
enase
nase
Acetyl CoA
FA
Increase in
fatty acid
UCP
CoASH
H
Cellular Actions
Acetyl-CoA
Acy
Acyl-CoA
yl(2 C Atoms
Atom Shorter)
PP
AR
RXR
ATP
A
ATP5J2
Cytosol
beta-Ketoacyl-CoA
thiolase
TCA
cycle
FADH2 /NADH
H+
ADP
P
Retinoic acid
repeat
NAD
AD+
NADH+H+
NADH
NAD
beta-Ketoacyl-CoA
Fatty acid
beta-oxidation
-
TAG
UCP
VLDL
3-Hyroxyacyl-CoA
H2O ACOT2
CoA
FAD
H2O
Enoyl-CoA
hydratase
CT
Mitochondrial
Matrix
Fatty acyl CoA
UCP
Acyl-CoA
dehydrogenase
enase
FADH2
FADH
F
trans-2-Enoyl-CoA
CPT2
FA-
Fatty acid
beta-oxidation
FACS
TAG TAG
T
TAG TAG
T
FA FACS
LPL
MLYCD
RXR
SLC27
AMPK
Acetyl CoA
Fatty
y acid
ATP
Acetyl-CoA carboxylase
Acyl-CoA thioesterase 2
ATP Synthase
Carnitine palmitoyltransferase
Carnitine acylcarnitine
translocase
Fatty acid anions
Fatty acyl CoA synthetase
Lipoprotein lipase
Malonyl-CoA decarboxylase
Retinoid X receptor
Solute carrier family 27
(Fatty acid transporter)
i.e. SLC27A1 & SLC27A6
Triacylglycerol
Uncoupling proteins
Very-low-density lipoproteins
ACAC
ACOT2
ATP5J2
CPT
CT
Fatty acid
H+
H+
IV
III
H+
H+
H+
II
PGC1 alpha
PPAR
I
H+
Discover more at abcam.com/fattyacidoxidation
Nucleus
Fatty Acid Uptake
i.e CD36
Fatty Acid
beta-oxidation
i.e CPT1
Fatty Acid Storage
i.e DGAT,SREBP
RXR
Response Element
PPAR Target Genes
Copyright © 2012 Abcam, All Rights Reserved.
Greater than 95% of all ATP production in the adult heart is derived from mitochondrial oxidative phosphorylation. Fatty acid
beta-oxidation makes up 50-70% of this ATP production. The remainder of ATP is derived from glycolysis and GTP formation
in the tricarboxylic acid (TCA) cycle. A number of enzymes involved in myocardial fatty acid metabolism are regulated largely
by nuclear receptor transcription factors including peroxisome proliferator activated receptors (PPARs) and PPAR gamma
coactivator 1 alpha (PGC-1α). In pathophysiological settings like obesity and diabetes, there is an increase of free fatty acid
in the cardiac myocyte which enhances the ability of PPARs to increase transcription of their target genes.
Antibody
Applications/Tests
Species Reactivity
ACAA1 [2H6BE7]
ACAA2 [EPR6732(2)]
ACADL [7F5DD6]
ACADM [3B7BH7]
ICC, IP, Flow Cyt, In-Cell ELISA
Flow Cyt, ICC/IF, IHC-P, WB
WB, IP
WB, IP, Flow Cyt, In-Cell ELISA,
IHC-P, ICC/IF
WB, ICC/IF, In-Cell ELISA, IHC-P
ICC, IHC-P, IP
Flow Cyt, ICC, IHC-P, In-Cell ELISA, IP
ICC/IF, WB
ELISA, IHC-P, WB
WB, ICC/IF, Flow Cyt
ICC/IF, WB
IP, ICC, In-Cell ELISA, Flow Cyt
WB, ICC/IF, In-Cell ELISA
WB
WB, IHC-P, ICC/IF
Flow Cyt, ICC/IF, IHC-P, In-Cell ELISA, IP
Flow Cyt, WB, IHC-P
WB
WB, ELISA, IHC-P
Flow Cyt, FuncS, IHC-Fr, WB
WB, IF, ELISA
ICC/IF, WB, IHC (PFA fixed), IHC-Fr, IP
IHC-P, WB, ICC/IF, IHC-Fr
IP, In-Cell ELISA, WB, Flow Cyt,
ICC/IF, IHC-P
ICC, IHC-P, Flow Cyt, IP
Flow Cyt, ICC, In-Cell ELISA, IP, WB
IHC-P, ICC/IF, IP, Flow Cyt, In-Cell ELISA
ICC/IF, IHC-P, Flow Cyt, WB, ELISA
ELISA, ICC/IF, WB
WB
WB
Rat, Cow, Hu
Hu
Hu, ms
Ms, Rat, Cow, Hu
ACADS [7E1AB5]
ACADVL [6A9AF2]
ACAT1 [9H10AB4]
Acetyl Coenzyme A Carboxylase [EP687Y]
ACOX1 - Aminoterminal end
ATP5A [7H10BD4F9]
CD36
Complex I Immunocapture [18G12BC2]
CPT1A [8F6AE9]
CPT1B [EPR7838]
Cpt1c
CPT2 [1C2AE6 ]
CROT
DGAT1
ERAB
FABP [L2B10]
FAT
Fatty Acid Synthase
Glutathione Peroxidase 1
HADHA [2C2AA5]
HADHA/HADHB [4A8BG12]
HADHB [5C3AB7]
HADHSC [1A12BC8]
Lipoprotein lipase [LPL.A4]
M Cadherin
MitoProfile® Total OXPHOS Antibody Cocktail
MitoProfile® Total OXPHOS Rodent
Antibody Cocktail
MLYCD
MTCO1 [1D6E1A8]
PGC1 alpha + beta
PPAR alpha
PPAR delta
PPAR gamma
Retinoid X Receptor [EPR7106]
SDHA [2E3GC12FB2AE2]
- Mitochondrial Marker
SDHB [21A11AE7]
SIRT1 [19A7AB4] - Nuclear Marker
SLC25A20
SLC27A1
WB, IHC-P, ICC/IF
ICC/IF, WB, Flow Cyt, IHC-Fr
WB
ELISA, ICC/IF, IHC-P, WB, IHC-Fr
ICC/IF, WB
WB, IHC-FoFr
ICC, WB, IP
ICC, IHC-Fr, Flow Cyt, WB, IHC-P
Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IF
IP, In-Cell ELISA, WB, Flow Cyt,
ICC/IF, IHC-P
WB, ELISA
ICC/IF, WB
Hu
Hu, Ms, Rat, Cow
Hu
Ms, Rat, Hu
Hu, Ms
Ms, Rat, Cow, Hu
Ms, Rat, Hu
Ms, Rat, Cow, Hu
Ms, Rat, Hu
Hu
Ms, Hu
Hu, Ms, Rat, Cow
Hu, Ms
Hu
Hu, Ms, Rat
Hu, Ms, Rat, AGMk, Cow
Fruit fly
Ms, Rat, Hu
Ms, Rat, Cow, Hu
Rat, Cow, Hu
ab110289
ab128929
ab128566
ab110296
ab110318
ab110285
ab110290
ab45174
ab59964
ab110273
ab64014
ab109798
ab128568
ab134135
ab87498
ab110293
ab103448
ab100982
ab52243
ab7366
ab20163
ab22759
ab22604
ab110281
Rat, Cow, Hu
Hu
Hu
Ms, Cow, Hu
Hu, Ms, Rat
Hu
Ms, Rat
ab110302
ab110301
ab110284
ab21356
ab65157
ab110411
ab110413
Ms, Rat, Cow, Hu, C.e, Zfish
Hu, Ms, Rat
ab14714
ab110304
Hu, Ms
Ms, Rat, Cow, Hu, C.e, Zfish
Ms, Rat, Hu
Hu, Ms, Rat
Hu, Ms
Hu, Ms, Rat
Hu, Ms, Rat
Ms, Rat, Cow, Hu, C.e
Hu
Hu
Key: AGMk - American Green Monkey | C.e - C.elegans | Gpig - Guinea pig | Hu - Human | Ms - Mouse | Shp - Sheep | Zfish - Zebrafish
Discover more at abcam.com/fattyacidoxidation
Product Code
ab95945
ab14705
ab72230
ab8934
ab8937
ab27649
ab125001
ab14715
ab82678
ab81875
Antibody
Applications/Tests
Species Reactivity
SLC27A6
SOD2 [9E2BD2]
SREBP1
SREBP2
Sterol carrier protein 2
Thioredoxin 2 [4C5]
UCP3 [EPR6427]
WB, ICC/IF
Flow Cyt, ICC/IF, IHC-P, In-Cell ELISA, IP
WB, ICC/IF
IHC-P, ICC/IF, WB
ICC/IF, WB
ELISA, IP
WB, IP, Flow Cyt
Hu
Hu, Ms, Rat, Cow
Ms
Ms, Gpig, Hu
Hu
Hu, Ms, Rat
Hu, Ms, Rat
Target
Kit
Species
ACADM
ARP4
ATP synthase
CD36
Coenzyme A Detection
Complex I Enzyme
Complex II Enzyme
Complex IV Enzyme
Dipeptidyl peptidase IV (DPP4)
Fatty Acid Oxidation
Fatty Acid Oxidation
Free Fatty Acid
Glutathione Reductase (GR)
Hydrogen Peroxide
Intracellular GSH
Lipid Hydroperoxide (LPO)
Lipid Peroxidation (MDA)
Lipoxygenase
Lysyl Oxidase (Fluorometric)
MitoBiogenesis™ (Colorimetric)
MitoBiogenesis™ (IR)
MitoTox™ Complete OXPHOS (5 Assays)
Myeloperoxidase (MPO)
Myeloperoxidase (MPO) (Colorimetric)
PAF Acetylhydrolase
PAF Acetylhydrolase
PicoProbe™ Acetyl CoA
PPAR (alpha, delta, gamma) Transcription Factor
PPAR alpha Transcription Factor
PPAR delta Transcription Factor
PPAR gamma Transcription Factor
SIRT1
SIRT1 (FRET-Based)
SREBP-1 Transcription Factor
SREBP-2 Transcription Factor
SREBP-2 Translocation
Thioredoxin (TrxR) Reductase
ELISA
ELISA
Enzyme activity microplate assay
ELISA
Quantitative assay
Enzyme activity microplate assay
Enzyme activity microplate assay
Enzyme activity microplate assay
Inhibitor Screening Assay
Flow cytometry
In-Cell ELISA
Quantitative assay
Quantitative assay
Enzyme activity assay
Flow cytometry
Quantitative assay
Quantitative assay
Inhibitor Screening Assay
Enzyme activity assay
In-Cell ELISA
In-Cell ELISA
Quantitative assay
Enzyme activity assay
Inhibitor Screening Assay
Enzyme activity assay
Inhibitor Screening Assay
Quantitative assay
Quantitative assay
Quantitative assay
Quantitative assay
Quantitative assay
ELISA
Quantitative assay
Quantitative assay
Quantitative assay
Quantitative assay
Enzyme activity assay
Ms, Rat, Hu
Hu
Hu, Rat, Cow
Mouse
Hu, Ms, Rat, Cow
Hu, Ms, Rat, Cow
Hu, Cow
Hu
Rat, Cow, Hu
Rat, Cow, Hu
Mammals
Mammals
Mammals
Ms, Rat, Cow, Hu
Ms, Rat, Cow, Hu
Cow
Hu
Mammals
Hu
Mammals
Ms, Rat, Hu
Mammals
Ms, Rat, Hu
Hu
Hu
Ms, Rat, Hu
Mammals
Ms, Rat, Hu
-
:MitoSciences product |
:RabMAbs product
Product Code
ab84183
ab110300
ab44153
ab72856
ab82382
ab16856
ab124925
Product code
ab129734
ab99974
ab109714
ab100673
ab102504
ab109721
ab109908
ab109909
ab133081
ab109880
ab118182
ab65341
ab83461
ab102500
ab112132
ab133085
ab118970
ab133087
ab112139
ab110217
ab110216
ab110419
ab133080
ab105136
ab133088
ab133091
ab87546
ab133113
ab133107
ab133106
ab133101
ab123457
ab133126
ab133125
ab133111
ab133125
ab83463
For additional products please visit our website
396_12_MBS
Myocardial Fatty Acid Metabolism