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MINERAL NUTRITION & ORGANIC SUBSTANCES CONCERNING DYE & FLAVOURS 2o Metabolites 1. DYE = PIGMENTS - Chlorophyll (green) - Carotenoids (yellow - red) - Xanthophyll (=oxygenated carotene) Carotene protect chlorophyll from Photodestruction (Albino turns green under dim light) & insect attractant in flower 2. Flavour = Aroma + Taste Aroma = essential oil / volatile oil = essence at room temp. - Alcohol, ether, aldehyde - Terpenoids Taste Sweet = glucose , fructose , sucrose ete. Sour = - organic acid (kreb’s cycle) (i.e. citric acid in lemon) - aromatic acid Pungent = i.e. capsaicin, piperine Bitter = alkaloids , terpenoid Astringent = tannin Pigments, odour & Taste substance are : o 1. 2 metabolite product after photosynthate occurred through metabolic pathway 2. waste material in vacuole Relation to Mineral Nutrition 1. Pn & Carbon skeleton Production 2. Enzyme synthesis & activities 3. Turgidity/Osmotic regulation 4. Product translocation 5. Energy supply for synthesizing and degradation 6. Stage of development & accumulation MINERAL NUTRITION & PHYTOHORMONE 1. AUXIN Metabolism - produced in active meristematic tissue (buds, young leaves & fruits) - immobilization by photo oxidation, enzyme oxidation (IAA - oxidase) - Peroxidation reduce Auxin activity (+O2) Plant response - Cellular effect : - increase DNA, RNA protein & enzyme - increase proton exchange & K uptake - influence phytochrome reaction to red far - red light - Necessary for callus growth (tissue culture, gall - , nodule tissue) - Rooting in plant cutting - Delay leaf & fruit abscission Agricultural use - 2,4 - D, 2, 4, 5 - T , MCPA (herbicide) - prevent fruit drop, induce ethylene formation (NAA) - thinning fruit for prevent biennial bearing - rooting compound - prevention of sprouting in potato Minerals & Auxin - IAA effect proton - pump by effect proton transport through cell wall (i.e. K+ uptake > Na+ > Li+) Salt resistant effect - Ca2+ necessary for Auxin - transport (Auxin & Ca2+ redistribution) - Zn2+ necessary for tryptophan synthesis 2. GIBBERELLINS Metabolism - biosynthesis in immature fruit & seed, bud, leaf & root - bound form in seed is freed through soaking, chilling - vernalization (seed) stratification (bud) increase GAs Plant response - act synergistically with auxin in apical dorminance, cambium growth, geotroprism, parthenocarpy, increase fruit - set - internode growth of dwarf plant - replace stratification Minerals & Gibberellin - GA3 increase membrane permeability, cause monovalent ion lost and transportation of ion from root - GA3 increase K+ transport - Zn & GA3 : positive correlation 3. CYTOKININ Metabolism : - produced in root - translocate through transpiration stream - buds stronger sink than leaf Pl. response - Adventitious bud formation (Cyt. + Auxin) - inhibit root initial formation in stem cutting (Auxin stimulate) - delay senescence (Chlorophyll retention, protein & amino acid retention) Mineral & cytokinin - effect on protein synthesis, cell wall & cell membrane synthesis and sugar metabolism - translocation of Fe from root - ion flux into root stimulate but ion efflux and water transport out of root reduce - N - application increase cytokinin synthesis in root - regulate ion secretion from salt gland Agricultural use - Control flowering, enhance growth & productivity - Enhance germination & emergence - Produce sterile male for hybrid seed industry (corn) - Seedless - amylase activity in malt industry 4. GROWTH INHIBITOR 3 groups : Nature 1) ABA 2) Benzoic - , phenolic acid & lactone (seed dormancy) Synthetic retardant 3) - ammonium salt (Amo - 1618), phosphon - D - SADH or daminozide - Chlormeguat chloride (CCC) - Morphactin (flurecol & chloro flurecol) Metabolism GA, ABA, cyt., chlorophyll, carotene & Xantophyll produced through “Mevalonic acid & isoprene pathway” Agricultural use - growth retardant SADH, CCC, Phosfon - D & morphactin - reduce lodging & vegetative growth - TIBA = IAA inhibitor (transport) - Defoliant before harvesting - inhibit axillary shoot development Pl. response to ABA - dormancy mechanism of bud & seed for survival - leaf abscission - GA/ABA ration in dormancy and germination after stratification - stomata closure - inhibit K uptake & proton extrusion - inhibit hydrolytic enzyme activity - inhibit flowering of long - day plant Minerals & ABA - inhibit K uptake into guard cell - inhibit K uptake reduce stomata close Amylase activity - effect ion transport, ion accumulation and selectivity of ion into preserve organ 5. Ethylene Metabolism - increase by Auxin application 2, 4 - D increase ethylene content in tissue upto 50 fold - increase in ripening & stress tissue Pl. Response - reduce elongation, increase stem diameter & ageotropic of seedling during germination - leaf & flower senescence (AgNO3 = anti ethylene activity) Agricultural use of Ethylene - Limit because of gas - form - Liquid form = ethephon - Suppress growth of seedling in starting bed = 10 days - Break seed dormancy Agricultural use - increase fruit - set & Fruit size (grape & apple) - Promote seed germination (Lettuce seed) - Tissue & pollen culture breeding End…