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What is Cellular Respiration? energy • When organisms release the _________ stored in carbohydrates and other food molecules Glucose Fructose Sucrose: A disaccharide Monosaccharides 3 stages of Cellular Respiration 1. Glycolysis: • • Occurs in the cytoplasm No O2 required 2. Krebs Cycle: • Occurs in the mitochondrion 3. Electron Transport Chain • Requires O2 Aerobic Respiration 1. Occurs when Oxygen is present 2. Many ATP Molecules made Anaerobic Respiration 1. Occurs when no Oxygen is present 2. Very little ATP made 3. Two basic types: a. Fermentation: Alcohol and CO2 produced b. Lactic Acid “Glyco” “lysis” literally means “sugar” “splitting” ATP ADP P ATP ATP ADP P = -2 Glycolysis takes place in the cytoplasm + P NADH NAD P P NADH NADP+ NADH = +2 Glycolysis takes place in the cytoplasm P ATP ADP P ATP = +2 ATP P ADP P Glycolysis takes place in the cytoplasm ATP ADP P ATP ADP P ATP = +2 Pyruvate What is the net gain of energy in Glycolysis? • ATP = + 2 • NADH = + 2 What did Krebs and Chance Discover in their Research? • Enjoy this video clip, and jot down a summary of Krebs’ and Chance’s research. • Pay attention to the significance of both scientist’s discoveries. Label the parts of the mitochondrion Inner membrane Outer membrane Cristae Matrix Intermembrane space The Krebs Cycle takes place in the Matrix of the Mitochondrion NADH NAD+ CoA Acetyl CoA NADH NAD+ CoA The Krebs Cycle takes place in the Matrix of the Mitochondrion CoA Citrate The Krebs Cycle takes place in the Mitochondrion NADH NAD+ The Krebs Cycle takes place in the Mitochondrion NADH NAD+ The Krebs Cycle takes place in the Mitochondrion ATP ADP NADH NAD+ P The Krebs Cycle takes place in the Mitochondrion FADH FADH2 What is gained and lost for each pyruvate molecule? • What is lost during the Krebs Cycle? – A Carbon atom must be lost – It is lost in the form of Carbon Dioxide – There are 3 CO2 molecules lost • What is gained during the Krebs Cycle? – 4 NADH molecules are made. – 1 FADH2 molecule is made – 1 ATP molecule is made What is gained per glucose molecule? NADH = +8 +4 ATP = +1 +2 FADH2 = +2 +1 • But there were two molecules of Acetyl CoA (per glucose) that went into the Krebs Cycle… • How many would actually be produced in this case? Where is the energy? • So far, most of the usable energy from the original glucose molecule is stored as NADH and FADH2 electrons in • Where will most of the ATP be made? The Electron Transport Chain Where is the Electron Transport Chain? Inside the mitochondrion. Many ATP molecules will be produced in these Folds of the inner membrane Electron Transport Chains known as cristae. How much ATP can each electron carrier make? ATP NADH ATP ATP FADH2 FADH2 FADH2 ATP ATP After electrons travel through the ETC, Oxygen is the final electron acceptor. Electrons, and two Hydrogen ions attach to the oxygen, and H2O is formed. Hydrogen ions pump from the matrix to the intermembrane space, using the energy from the electrons. The diffusion of H+ ions through the enzyme, ATP synthase makes ATP C6H12O6 ATP C6H12O6 CO2 H2O ATP C6H12O6 + O2 CO2 + H2O + ATP Photosynthesis and Respiration • With a neighbor, fill in the steps in this cycle of Photosynthesis and Respiration. • We will check your answers when you are done!