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Objective for today Metabolism Photosynthesis Cellular Respiration Metabolism What is metabolism? Why do we need all those chemical reactions? The sum of all chemical reactions occurring in the body. There are lots of jobs that must be done to keep us alive! Breathing, digestion, movement, thinking, immunity, growth, etc. All those reactions require energy. Where do we get the energy from? ENERGY: We get the majority of our energy from our FOOD Energy is released from the GLUCOSE in food Every time a bond is broken ENERGY is RELEASED Glucose is broken down into 2 molecules of pyruvic acid in the cytoplasm of the cell 2 Molecules of Pyruvic Acid move into the mitochondria and are broken down into Carbon Dioxide 2 Laws of Thermodynamics 1st Law: Energy cannot be created or destroyed, but it can change from one form to another. Solar, chemical, mechanical, heat energy, etc. 2nd Law: When energy changes forms, some of the energy is wasted or lost as… HEAT! Living things depend on the Sun! (wasted energy) (wasted) (stored in glucose) (wasted) (Used for Movement) Two Types of organisms Autotrophs Make their own energy containing molecules Two types: Photosynthetic: use the sun Chemosynthetic: use inorganic chemical reactions Heterotrophs Can’t make their own food; do “take out” Photosynthesis Solar energy + 6CO2 + 6H2O C6H12O6 + 6O2 (carbon (water) dioxide) (glucose) (oxygen) Reactants: solar energy, carbon dioxide and water CO2 and H2O diffuse into the plant cell. Products: high energy glucose and oxygen Photosynthesis transforms solar energy into the chemical energy of a carbohydrate. The photosynthetic process takes place with organelles called chloroplasts. Photosynthesis: 2 sets of reactions Light reactions Solar energy Chemical energy (ATP, NADPH) Calvin Cycle reactions (aka Dark reactions) Chemical energy Chemical energy (ATP, NADPH) (carbohydrate) C6 H12 O6 Sun + 6CO2 + 6H2O C6H12O6 + 6O2 Solar energy can be converted to chemical energy. Spot the Dog: See Spot Run This chemical energy can be stored in the covalent bonds that hold together the atoms in a molecule of glucose. Chemical energy can be converted to mechanical energy. See Spot run! Chemical energy is stored in each of these covalent bonds. Before an organism can use the chemical energy in glucose, the energy must be stored in ATP. ATP is the “currency” of cellular energy. ATP directly supplies the energy necessary for nearly all chemical reactions in the body. ATP: Adenosine Triphosphate nucleotide Very high energy bonds between phosphate groups (like charges repel) ATP Chemical energy is stored in the high energy bonds that hold the phosphate groups together in the ATP molecule. When ATP is broken down, a phosphate group is released along with free energy that can be used for metabolism. Functions of ATP Chemical work: ATP supplies the energy needed to build organic molecules in the cell. Transport work: ATP supplies the energy to pump substances across the cell membrane. Mechanical work: ATP provides energy for movement. (muscle contraction, flagella, chromosome movement during mitosis, etc.) Exergonic = releases energy So how & where is ATP formed? Outside the mitochondria… Glycolysis: Glucose (6 carbons) is converted to pyruvate (3 carbons) Energy input 2 ATP 4 ATP made net gain of 2 ATP P.S. Don’t memorize this diagram! (glyco = sugar; lysis= breakdown) Inside the mitochondria… Krebs cycle (aka Citric Acid cycle or TCA cycle) Big Picture: Organic molecules from glycolysis enter the mitochondria. These molecules are processed in the Krebs cycle. The products of the Krebs cycle are: • Carbon Dioxide (waste product) • ATP (used for energy) • etc. •Net gain of 2 ATP Don’t memorize this diagram! Cellular Respiration Cellular respiration is the process of breaking down glucose to produce carbon dioxide , water and ATP. Aerobic respiration requires oxygen and produces 32 ATP molecules per glucose. Anaerobic respiration (aka fermentation) does not require oxygen. It is a less efficient process than aerobic respiration, producing only 2 net ATP. The products of fermentation include either lactate or alcohol. When our muscles need more oxygen than they are supplied, cellular respiration becomes anaerobic. Lactic acid is released as a waste product. Fermentation (AKA anaerobic respiration) What happens to the products of the Krebs cycle? ATP is used as an energy source. Carbon dioxide exits the cell by diffusion. Eventually, it will diffuse into the bloodstream. After that, CO2 will diffuse from the bloodstream into the alveoli of the lungs. Then, it is released into the atmosphere by exhalation. Plants will absorb the carbon dioxide to use in the photosynthetic process all over again. Big Picture Organisms require energy for metabolism. That energy ultimately comes from the sun’s energy stored in the chemical bonds of glucose, produced by photosynthesis. Mitochondria within cells have the machinery necessary to turn that glucose into ATP that the cell can use for energy. Living things depend on the Sun! (wasted energy) (wasted) (stored in glucose) (wasted) (Used for Movement) Hmmmmmmmmm Photosynthesis CO2 + H2O + sunlight energy > glucose + O2 Cellular respiration Glucose + O2 > CO2 + H2O + ATP energy Bromothymol Blue Used to test for the presence of carbon dioxide (CO2) If CO2 is present it is a yellow color If CO2 is NOT present it is a blue color