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Size (MW)
?
Unidentified protein
extracted from gel
Isoelectic Point (pI)
Digestion of proteins into
fragments of 5-10 amino
acids
Separation of proteins
by 2 DE
MDISTLTASEEIEMI
DAEEIEMATEDILAE
DLISLFMIDDMFSSI
DLESISFIFNSSDIDSI
EDENIDLESIEEIEM
FIEEIEMATIFNSSED
IDSMMOSINFEIFNS
SDIDMMDATIDLAE
D LIS LFM DDM FSS
++++
…..AEDLISLFMDDM…..
Sequence
Database
Determine mass of the fragments
using a mass spectrometry ( MS)
Determine amino acid sequence
and compare with sequence
database
Protein identified
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