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http://en.wikipedia.org/wiki/Lysozyme
Lysozyme
A.k.a :
Muramidase OR
N-acetylmuramide glycanhydrolase
Lysozyme
An enzyme found naturally in
• egg white
• human tears
• saliva (as well as other body fluids)
Capable of destroying the cell walls of certain
bacteria.
Acts as a mild antiseptic.
Amino Sequence
Fig 5. Lysozyme amino acid sequence alignment for five organisms (Dmelanogaste-fly, EcoligsnAA-E. coli,
GgallusgsnAA-chicken, HsapiensgsnA-human, and Mmusculusgsn-mouse). Number to the left and right of the
sequences and above each sequence group indicate amino acid number. Letters represent amino acids, with
dashes (-) inserted to maximize sequence alignment. Black boxes indicate amino acid conservation.
Structure
Made with 117 amino acid residues and
has a heterogeneous sequence.
1REM
133L
1IWV
<http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1rem#>
Quaternary
Structure (4°)
Function
Lysozyme attacks peptidoglycans and hydrolyzes the
glycosidic bond that connects N-acetylmuramic acid with
the fourth carbon atom of N-acetylglucosamine. It does
this by binding to the peptidoglycan molecule in the
binding site within the prominent cleft between its two
domains.
Peptidoglycans- sugars & amino acids that form a meshlike layer outside the plasma membrane of bacteria,
forming the cell wall.
Hydrolysis- Decomposition of a chemical compound by
reaction with water, such as the dissociation of a
dissolved salt or the catalytic conversion of starch to
glucose.
http://en.wikipedia.org/wiki/Lysozyme
Present in other Organisms
• bacteriophages (viruses that infect bacteria)
• bacteria
• plants
• animals
Misc…
• Lysozyme was first identified and named by
Alexander Fleming, who recognized its
bacteriolytic properties. (1922)
• Bottlefed babies are 3 times likely to have
diarrheal disease, because they lack lysozyme
in their diet... [its in breast milk!] Lysozyme is a
natural form of protection from pathogens.
Bacteriolysis -destruction or dissolution of bacterial cells
Works Cited:
1.
Yikrazuul. "Lysozyme." Wikipedia. N.p., 14 Oct. 2009. Web. 14 Oct. 2009. <http://en.wikipedia.org/
wiki/Lysozyme>.
2.
Muraki, M., et al. "HUMAN LYSOZYME WITH MAN-B1,4-GLCNAC COVALENTLY ATTACHED TO ASP53."
RCSB Protein
Data Bank. N.p., 14 Oct. 2009. Web. 14 Oct. 2009. <http://www.rcsb.org/pdb/cgi/
explore.cgi?pdbId=1rem>.
3.
Secretlens. "Drool." DeviantArt. N.p., 15 July 2009. Web. 14 Oct. 2009.
<http://secretlens.deviantart.com/art/Drool-129635147>.
4.
funkipunk . “Tears Speak." DeviantArt. N.p., 13 Nov. 2007. Web. 14 Oct. 2009.
<http://funkipunk.deviantart.com/art/Tears-Speak-69773894>.
5.
Caothicart . “Dark Pikachu." DeviantArt. N.p., 10 June 2007. Web. 14 Oct. 2009.
<http://caothicart.deviantart.com/art/Dark-Pikachu-57318376>.
6.
Chueh, Luke . “I ASKED FOR SCRAMBLED.” LukeChueh N.p., 2003. Web. 14 Oct. 2009.
<http://www.lukechueh.com/paintings/i-asked-for-scrambled.html>.
7.
Stryer L. 1995. Biochemistry. 4th ed. New York: W.H. Freeman and Company. p 372.
8.
"MacDNAsis Analysis of Lysozyme Genbank search results." Davidson College. N.p., 30 Nov. 2001. Web.
14 Oct. 2009. <http://www.bio.davidson.edu/Courses/Molbio/MolStudents/01grnoland/
MacDNAsis.html>.