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Transcript 
MES-4
Chromatin Regulation in the C.
elegans Germ Line
Dosage compensation in worms:
XO
XX
soma
both Xs are
reduced ~50%
germline
Xs are not
transcribed
How chromatin is organized…
The Histone Code
N
N
N
N
H4
H4
H3
H3
acetylated
methylated
methylated
Lys 8, 12, 16
Lys 27
Lys 20
activating
silencing
silencing
N
N
methylated
Lys 9
silencing
methylated
Lys 4, 36
activating
H2A
The nucleosome.
Get better resolution!
H3
H2B
H4
Ref.
Kornberg and Lorch, Cell 1999
MES-4 binds to just 5 of the 6 chromosomes.
maternal
pronucleus
MES-4 + DNA
DNA alone
paternal
pronucleus
H3-Lys36 di-methylation is on Xs in early embryos.
MES-4 is required for H3-Lys36 di-methylation.
1-cell
WT
2mK36
WT
mes-4
mes-4
Chromatography
Sucrose Gradients
Gel Electrophoresis
Antibody Use in Detection of Target Protein
MES-4 and its homologs
NSD1 catalyzes H3 Lys36 and H4 Lys20 methylation in vitro.
(Raysam et al., EMBO J 2003)
C. elegans (MES-4) and C. briggsae (CBG60706)
Homologs
MES-4 and CBG60706
CB60706 and MES-4 Introns and Exons
Nucleotide Alignment
BLAST Amino Acid Alignment Under Default Settings
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