Download Prezentacja programu PowerPoint

Humoral and Cellular
Response Against Native
and Synthetic Antigens.
Institute of Maritime and Tropical
Medicine
Department of Immunology &
National Salmonella Centre
2017-05-23
1
Department of Immunology
& National Salmonella Centre

Human Resources:
- Full professor: Renata Głośnicka MD, Ph.D.
- Senior scientists: Danuta Kunikowska Ph.D.
Bożena Dera-Tomaszewska Ph.D.
Jan Wysocki MD, Ph.D.
- Scientists: Halina Dziadziuszko M.Sc.
Ewa Tokarska-Pietrzak M.Sc.
2017-05-23
2
OUR TEAM:
From centre to left
(clockwise):
Prof. Renata Głośnicka,
Dr. Bożena DeraTomaszewska, Jolanta
Zabłocka, Dorota Kenig,
M.Sc. Halina
Dziadziuszko, Gabriela
Nawrot, M.Sc. Ewa
Tokarska -Pietrzak, Dr.
Danuta Kunikowska
2017-05-23
3
PREVIOUS RESEARCH

Immunochemistry research:
- Salmonella bacteria antigens
- Lipopolysaccharides
- Flagella
- Vi antigen
- Yersinia pestis envelope antigen
2017-05-23
4
Immunochemistry Research


Lipolysaccharide of Salmonella
Haarlem (9.46) – detection the new
OPS composition: 3,9,46 (according
to Kauffmann-White scheme).
Epitope analysis of S. Enteritidis
isolated flagella antigen and synthetic
peptides of hypervariable part of this
antigen.
2017-05-23
5
Immunological investigations of
viruses synthetic peptides.


Hepatitis B Virus:
- X-Gene coded protein fragments;
- surface antigen (140-146) region fragment.
Hepatitis C Virus:
- protein fragments
(evaluation of their immunogenicity and
diagnostic propertis in HCV infection)
2017-05-23
6
Immunological investigations of
heat shock protein fragments.


Human hsp 70 fragment (29-42), and
their cyclic analogue – conformational
studies and evaluation of its
immunogenicity.
Human hsp 60 fragment (409-424) – is
recognized by serum antibodies of patients
with acute coronary syndroms.
2017-05-23
7
Immunological investigations of heat shock
protein.

Characterisation of the anti-DnaJ
monoclonal antibodies and their use to
compare immunological properties of
DnaJ and its human homologue HDJ-1 –
epitope analysis of the similar domains of
E. coli DnaJ and human HDJ-1 (in
cooperation with Prof. B. Lipińska team,
UG).
2017-05-23
8
CURRENT RESEARCH
National Salmonella Centre
- diagnostics of Salmonella strains isolated
by field laboratories
- molecular research of Salmonella strains
- epidemiological research within WHO
„Salmonella Surveillance” project
2017-05-23
9
Salmonella infections among people in the years
1971-2000
100000
Number of cases
90000
80000
70000
Salmonella
S.Enterit.
other Salm.
60000
50000
40000
30000
20000
10000
0
2017-05-23
YearYear
10
CULTURE COLLECTIONS
• Salmonella strains ( KOS Collection - about 2000 strains)
• Bacteriophage preparations
• Mouse myeloma cell lines
• Mouse macrophages cell line
• Mouse hybridoma cell lines producing monoclonal antibodies
anti Salmonella antigens and anti DnaJ from E.coli.
2017-05-23
11
POLYVALENT VACCINE „IMMUNOVAC”
AGAINST SALMONELLOSIS IN ANIMALS



„Immunovac” vaccine is composed of
several Salmonella serovars and polyclonal
antibodies
Generates active and passive immunization
after oral administration.
The vaccine is in the course of
commercialization by BIOWET in Puławy.
2017-05-23
12
IMMUNOLOGICAL INVESTIGATIONS
OF PORPHYRIN DERIVATIVES
Porphyrines and their amino acids derivatives
• PP(FAla)2Arg2
• PP(Ala)2Arg2
• PP(Ser)2Arg2
and their mixture (Sensifirine)
are intended to be used in PDT (photodynamic
therapy).
Our goal is to investigate their impact on the
immune system.
The researches are performed in cooperation with
the teams of prof. Alfreda Graczyk (MUT,
Warsaw) and prof. Anna Podhajska ( MBW AMGUG, Gdańsk)
2017-05-23
13
ANTIBODIES
&
ANTIGENS
Y
Y
HOW DO THEY WORK ?
MOLECULES
2017-05-23
MEDICINE
14
THANK YOU
FOR YOUR ATTENTION
2017-05-23
15