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MIT Department of Biology
7.013: Introductory Biology - Spring 2005
Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel
7.013 Spring 2005
Recombinant DNA terms
Recombinant DNA - Terms to know
Polymerase Chain Reaction (PCR)
DNA Sequencing: dideoxy chain termination – end label and fluorescent dideoxy
methods
Genotyping
SNPs
Polymorphisms (RFLP)
Gene therapy (ex-vivo and in-vivo)
Retroviral vector
Genetically engineered animal models
Gene targeting
Knock-out mice
Transgenic mice
Promoter
Plasmid
Origin of Replication (ORI)
Drug resistance (e.g. Amp)
Multiple cloning site
Restriction enzyme
Hybridization
Cloning by complementation
Oligonucleotide probe/primer
Precursor RNA
mRNA
splicing
DNA polymerase (Taq)
Reverse Transcriptase (RT)
Library (cDNA, genomic)
Hybridization
Anneal
Transformation
Bacterial colonies
Ligation
DNA ligase
Denaturation
Agarose Gel Electrophoresis
Genetic Engineering
Cloning
Recombinant DNA revolution
DNA fragment
Palindrome
Sticky ends
Blunt ends
EcoRI recognition sequence
DNA amplification
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