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Transcript 
BioMEMS: Microfluidics Optimization
& Cell Culture Maintenance
D. Michael Ackermann, John Payne, Hillary Samples, James Wells
Group # 23
Advisor: Franz Baudenbacher, PhD.
The Problem: MEMS bioreactor
• Design Bioreactor
– Accurate nano/picoliter fluid delivery
– Structure for cell culture
• Bioreactor Maintenance System
– Temperature control
– Metabolic Measurements
– Photographic surveillance
The Bioreactor
• Cell culture structure:
•
Research Defined
Fluid Delivery
– Nutrient Delivery –
Syringe Pumps
– Drug/Toxin Delivery –
MEMS pumps
Bioreactor: The Plan
• Literature Review
Fall
– Picoliter Pump Designs
– Cell Culture Structure
• Training/Analysis of Current Designs
– Microfluidic Profiling (External Pumps)
• Design MEMS Pump
• Implementation into Reactor
• Analysis/Design Correction
Jan
Feb
Mar
BioReactor: Current Work
• MEMS Fabrication
– Expose and Develop
Photo-resist
– Pour PDMS onto
template
– Attach PDMS to slide,
making channels
The Maintenance System
• Temperature Control
Integrated Heating Block
• Provide CO2 Incubation
Maintain Viable pH Level
• Monitor Metabolic Activity
pH, glucose levels, etc
• Real-time Photography
LabVIEW Image Acquisition Builder
Maintenance System: The Plan
• Cell Culture Background & Training
Fall
– NanoDelivery, Inc. Laboratory
– Splitting, re-Suspension, Medium Change, Microscopy, etc
• Incorporate System Inputs
Jan
Heater, CO2 Line, Injection scope
• Implement Measurement Acquisition Systems
• Design Image Acquisition Program
Jan
Feb
LabVIEW
Real-Time photography
-Cellular responses & Maintenance in Bioreactor
• Analysis
Controlled Delivery
Mar
Current Work:
Bioreactor Maintenance System
Learning Background and Technique
Grow Cells
Cell Splitting
Change medium
Delivery into
bioreactor
Maintenance for
future generation
cell line
Total Integrated System
BioReactor
+
Maintenance System
Controlled Environment
Controlled Input
Measurement System
Obstacles
• Communication with advisor
• Website – access denied!
• Training Requirements
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