Download Lecture 19 - Buffalo State College

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

page
1
page
2
page
3
page
4
page
5
page
6
page
7
page
8
page
9
page
10
page
11
page
12
page
13
page
14
page
15
page
16
page
17
page
18
page
19
page
20
page
21
page
22
Document related concepts
no text concepts found
Transcript 
Lecture 21
HPLC-2
Instrumentation
Injector
Column
Chromatograph
Data Processing
Detector
Percentage of solvents having the same eluent strength
B-
acetonitrile CH3CN
t r2
t r2
N  16  2  constant 
 constant
2
W
W
tr
 constant
W
Width W is proportional
to retention time tr
‘general chromatography problem’
Solution of GNP: gradient
Pump
4.5 mm2 HPLC columns of different length
Injector
Detectors
UV cell
Derivatization in HPLC
Evaporative LightScattering Detector
Electrochemical detection of alcohols in water
HO
1
OH
HO
2
3
CH3OH
4
HO
HO
CH3CH2OH
OH
5
OH
6
OH
OH
7
OH
HO
13
O
14
8 2-butanol
9 2-Methyl-1-propanol
10 1-butanol
11 3-methyl-1-butanol
12 1-pentanol
Chemistry: Oxidation of alcohols @Au electrode
OH
MS Detector
Related documents