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PicoFill-Systems Outlicensing Electrospray Deposition onto Small Targets Spraying of Uniform Dots: two methods 1. Preferred method: spraying on a target followed by an increase of the spraydistance to reduce the electric field which forced the spraying to stop immediately. At the same time the chip is moved in x or y direction to position the second target underneath the capillary-tip. Finally, the spray-distance is reduced and spraying resumes immediately. In-between spraying of dots, the pump may run continuously. 2. In-between spraying of dots, the electric field can be reduced or turned of by programming the High Voltage power supply. However, this should be synchronized with the x-y movement of the chip. 1 Spraying of Arrays of Dots A. Piston pushes liquid through syringe while electric field is applied B. Slide is moved downward to lower the electric field: spraying stops 1–2 kV Spray of droplets MicroArray or slide C. Slide is moved upward and spraying resumes 1-2 kV 1-2 kV Spot Slide is moved downward and sideways: typical 200 – 1000 micron Second spot is sprayed onto slide 2