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Photosynthesis
Energy & Life
• Energy, energy,
ENERGY!
• Autotrophs vs.
heterotrophs
Chemical Energy & ATP
• One of the principle
compounds that cells use to
store and release energy is:
– Adenosine triphosphate (ATP)
• Storing energy:
– ADP + P
• Releasing energy:
– Breaking the bond between
the 2nd and 3rd phosphate.
• Active Transport, protein
synthesis,
movement…..firefly?
Investigating Photosynthesis
• van Helmont:
– Soil, seedling, and water
• Priestley:
– Candle, jar, sprig of mint
• Ingenhousz:
– In the absence of light….
Photosynthesis: An Overview
• Plants use the energy of sunlight to convert water
and carbon dioxide into high energy sugars and
O2, a waste product.
• Requires sunlight, water, and carbon dioxide.
• Overall equation:
6 CO2 + 6 H20  C6H12O6 + 6 O2
• Occurs in the leaves of plants in organelles called
chloroplasts.
Leaf Structure
• Photosynthesis occurs (mostly) in the palisade layer.
• Gas exchange of CO2 and O2 occurs at openings called stomata
surrounded by guard cells on the lower leaf surface.
Chloroplast Structure
• Inner membrane
called the thylakoid
membrane.
• Thickened regions
called thylakoids. A
stack of thylakoids is
called a granum.
(Plural – grana)
• Stroma is a liquid
surrounding the
thylakoids.
Pigments
• Chlorophyll a is the most
important photosynthetic
pigment.
• Other pigments are also
present in the leaf.
– Chlorophyll b
– Carotenoids (orange / red)
– Xanthophylls (yellow / brown)
• These pigments are
embedded in the membranes
of the chloroplast in groups
called photosystems.
Light = energy! Any compound that absorbs light also
absorbs energy. Chlorophyll absorbs light and transfers
that energy to electrons in the chlorophyll molecule:
LET PHOTOSYNTHESIS BEGIN!!!!
Photosynthesis: The Chemical Process
• Occurs in two main phases.
– Light reactions
– Dark reactions (aka – the Calvin Cycle)
• Light reactions are the “photo” part of
photosynthesis. Light is absorbed by pigments.
• Dark reactions are the “synthesis” part of
photosynthesis. Trapped energy from the sun is
converted to the chemical energy of sugars.
Light Reactions
• Light-dependent reactions occur on the
thylakoid membranes.
– Light and water are required for this process.
– Energy storage molecules are formed. (ATP
and NADPH)
– Oxygen gas is made as a waste product.
Dark Reactions
• Dark reactions (light-independent) occur in the
stroma.
– Carbon dioxide is “fixed” into the sugar glucose.
– ATP and NADPH molecules created during the
light reactions power the production of this
glucose.
The Reactions of Photosynthesis
• Follow along with handout
The Reactions of Photosynthesis
Factors Affecting Photosynthesis
• Water
• Temperature
• Light intensity