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Figure S4
a
WT Mock
WT+BTH
782+BTH
784+BTH
50
Disease index(%)
b
*
*
40
I3+BTH
*
*
*
*
30
*
*
20
*
10
0
WT Mock
c
I1+BTH
WT Mock
0 48 48hpi
WT+BTH
782+BTH
784+BTH
I1+BTH
I3+BTH
WT+BTH
782+BTH
784+BTH
I1+BTH
I3+BTH
0 48 48hpi 0 48 48hpi 0 48 48hpi 0 48 48hpi 0 48 48hpi
OsNPR1
Actin
Figure S7 OsNPR1 is involved in BTH-induced blast resistance in rice. WT is the wild-type
untransformed rice Gui99. The numbers 782 and 784 represent two independent Gui99 transgenic
OsNPR1-overexpressing lines. The I1 and I3 are two independent Gui99 OsNPR1-RNAi lines.
a Disease symptom of rice plants after infection with M. oryzae strain CHL0742.
The three-week-old rice seedlings were sprayed with 10 mM BTH (the commercial product Bion,
Ciba-Geigy GmbH, Germany). The mock treatment was foliar spray with distilled water. The plants
were inoculated with M. oryzae 48 h after treatment. The photograph was taken 7 days post
inoculation.
b Average disease index of the rice plants. Data are the means ±SD from three repeats, each using
10 leaves. Statistically significant differences are indicated by * (P <0.05) and ** (P <0.01). BTHtreated WT was compared with mock-treated WT. Each of BTH-treated OsNPR1-overexpressing
lines was compared with BTH-treated WT. Each of BTH-treated OsNPR1-RNAi lines was compared
with BTH-treated WT.
c Northern blot analysis of the expression of OsNPR1 in rice plants. Total RNAs were extracted from
rice leaves 0 h and 48 h after BTH treatment and also from leaves 48 h post inoculation (hpi).