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The Carbon Sink Strength of Beech in a Changing
Environment: Experimental Risk Assessment of
Mitigation by Chronic Ozone Impact (CASIROZ)
Contribution of the University of Freiburg
Prof. Dr. H. Rennenberg
Dr. K. Haberer, M. Alexou
(participant 4, WP1)
Albert-Ludwigs-Universität Freiburg i.Br.
Institut für Forstbotanik und Baumphysiologie
Georges-Köhler-Allee
D-79085 Freiburg
In association:
PD Dr. C. Langebartels (subcontractor 2, WP2)
Institute for Biochemical Plant Pathology
GSF-Forschungszentrum für Umwelt und Gesundheit
D-85764 Oberschleißheim
Project structure
GENERAL PUBLIC
ENVIRONMENTAL POLICIES
DECISION MAKING
WP 2
3 CR
Subcontractor1
WP 1
1 CO
2 AC
6 CR
4 CR
7CR
8CR
Subcontractor 2
5CR
Workpackage description
WP 1
WP 2
Ecophysiology and modeling
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Transpiration, stomatal conductance,
photosynthesis
Relative activity changes between
carbon fixing enzymes
Control of carbon fixation at the gene
level
Stem production, xylem mass flow
through the trunk
C and N allocation in phloem and
xylem (cycling of carbohydrates,
N-Status)
Fine-root functionality (H2O
acquisitation)
Identification of different types of
ectomycorrhiza
Fumigation experiments in cuvettes
Exposure of young beech plants in the
canopy
Biochemistry and molecular biology
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Production of defense metabolites in
leaves:
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Antioxidants and redox status
Injury amplification reactions
Regulation of defense reactions
at the gene level
Genetic regulation of Rubisco, PEPC
Production of defense metabolites in
roots
Examination of chromosomes
Phytohormonal relationships in
leaves, roots, phloem, xylem
Extent of mycorrhization
Examination of 3-year-old beech
plants
MODELING
Aims of the project –part of CR 4/sub-c 2-
Effects of chronic O3-exposure on
Activity of carbonfixing enzymes
N-Status
Level of sugars
Cycling of
carbohydrates
Antioxidative
system
Injury
amplification
reactions
Phytohormons
Fine-root
functionality
Hypotheses
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Risk of O3 injury to processes of primary metabolism
under light limitation is as high as under sunlight.
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The symptom development in beech leaves as a
consequence of O3 exposure are determined by the
antioxidant status and damage amplifying reactions.
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Effects of chronic O3 exposure on processes of primary
metabolism are different in young and adult trees and
cuvette fumigation experiments.
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Chronic O3 impact constrains the carbon sink strength.
Role of participant 4 and sub-contractor 2
adult trees
young trees
cuvettes
workgroup
workgroup
workgroup
Carbon-fixing enzymes/
WUE (12C/13C and
16O/18O ratio)
H. Rennenberg
H. Rennenberg
Antioxidants:
Ascorbate/Glutathione
H. Rennenberg
M. Tausz
H. Rennenberg
Antioxidants:
Tocopherol/Carotinoids
M. Tausz
M. Tausz
M. Tausz
C. Langebartels
C. Langebartels
C. Langebartels/D.
Hanke
C. Langebartels/D.
Hanke
Stress defense at gene
level
C. Langebartels
C. Langebartels
Sugar concentration
xylem/phloem
H. Rennenberg
H. Rennenberg
Translocation xylemphloem (14N/15N)
H. Rennenberg
H. Rennenberg
Fine-root functionality
H. Rennenberg
Examinated factors
Ethylen, ROS, salicylic
acid
Phytohormons
Methods
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Examination of carbon-fixing enzymes
( water-use-efficiency)
12C/13C
and
16O/18O
IR-MS
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Ascorbate
High Pressure Liquid
Chromatography (HPLC)
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Glutathione
High Pressure Liquid
Chromatography (HPLC)
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Sugar concentration
High Pressure Liquid
Chromatography (HPLC)
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N-status/translocation xylem-phloem
TSSN-analysis
14N/15N (IR-MS)
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Fine root functionality (H2O-acquisitation)
16O/18O
(Isotope Ratio Mass Spectrometry)
(IR-MS)
Time table
march
2003
Methodological
preparations
march
2004
Dataset adult and
young trees
st
(1 growing season)
march
2005
Dataset adult and
young trees
nd
(2 growing season)
Field assessment:
Sampling, analysing leaves, xylem, phloem, stem
wood, soil
Data integration