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CELL STRUCTURE & FUNCTION • The Cell Theory: – The Cell is the Simplest Unit of Life – All Organisms Are Made of One or More Cells – All Cells Come From Pre-Existing Cells • The Basic Features of All Cells – – – – Barrier: Plasma Membrane – Fluid Mosaic Cytoplasm: Interior of the Cell Energy Metabolism: – Energy & Nutrients from Outside Hereditary Information: – DNA • The Common Properties of All Cells – Same Molecules – Same Structures – Energy Metabolism - Electron Transport System Copyright © 2005 Pearson Prentice Hall, Inc. T5.1 CELL STRUCTURE & FUNCTION – Cell Function Limits Cell Size • Cell surface area to volume ratios (p. 80) • Relative sizes (F5.1 p. 77) • A comparison of microscope images 79) Microscopes yesterday and today (FE5.2 p. Copyright © 2005 Pearson Prentice Hall, Inc. (FE5.1 p. 78) Diameter 100 m tallest trees 10 m 10 cm visible with unaided human eye 1m adult human chicken egg 1 cm frog embryo 100 µm 10 µm 1 µm 10 nm 1 nm visible with special electron microscopes 100 nm most eukaryotic cells visible with conventional electron microscope visible with llight microscope 1 mm mitochondrion most bacteria virus proteins diameter of DNA double helix atoms 0.1 nm Units of measurement: 1 centimeter (cm) = 1/100 m 1 meter (m) = 39.37 inches 1 millimeter (mm) = 1/1000 m 1 micrometer (µm) = 1/1,000,000 m 1 nanometer (nm) = 1/1,000,000,000 m Paramecium cilia cilia nucleus mitochondrion contractile vacuole Light microscope 50 micrometers Transmission electron microscope 0.5 micrometers mitochondria Scanning electron microscope 10 micrometers Scanning electron microscope 5 micrometers CELL STRUCTURE & FUNCTION cilia mitochondrion 0.5 micrometers Electron microscope CELL STRUCTURE & FUNCTION • There Are Two Basic Types of Cells: Prokaryotic and Eukaryotic – A generalized prokaryotic cell (F5.2 p. 80) – Cell Structures, Their Functions, and Their Distribution in Living Cells (T5.2 p. 81) – A generalized animal cell F5.3(p. 82) – A generalized plant cell (F5.4 p. 83) Copyright © 2005 Pearson Prentice Hall, Inc. chromosome (nucleoid region) pili ribosomes food granule prokaryotic flagellum capsule or slime layer cell wall plasmid (DNA) cytoplasm plasma membrane flagellum nuclear pore chromatin (DNA) nucleolus nuclear envelope nucleus centriole intermediate filaments rough endoplasmic cytoplasm reticulum ribosome plasma membrane lysosome Golgi complex microtubules Smooth endoplasmic reticulum free ribosome mitochondrion vesicle vesicle Microtubules (part of cytoskeleton) plastid mitochondrion chloroplast central vacuole Golgi complex smooth endoplasmic reticulum vesicle plasmodesma cell wall rough endoplasmic reticulum plasma membrane nucleus nucleolus nuclear pore chromatin nuclear envelope intermediate filaments ribosomes free ribosome CELL STRUCTURE & FUNCTION? • Major Features of Prokaryotic Cells – Small – Specialized Surface Features – Few Specialized Cytoplasmic Structures Copyright © 2005 Pearson Prentice Hall, Inc. CELL STRUCTURE & FUNCTION • Major Features of Eukaryotic Cells Eukaryotic Cells Contain Organelles – Nucleus: Control Center (F5.5 p. 84) – Nuclear Envelope: Selective Exchange of Materials – Chromatin: DNA - Codes for the Synthesis of Proteins - Chromosomes (F5.6 p. 84) – The Nucleolus: Site of Ribosome Assembly • Ribosomes • Endoplasmic reticulum Copyright © 2005 Pearson Prentice Hall, Inc. (F5.7 p. 85) (F5.8 p. 85) nuclear envelope nucleolus nuclear pores chromatin nucleus nuclear pores chromatin chromosome ribosomes rough endoplasmic reticulum 0.5 micrometer vesicles smooth endoplasmic reticulum 0.5 micrometer vesicles from ER Golgi complex vesicles leaving Golgi complex CELL STRUCTURE & FUNCTION – The Travels of a Secreted Protein – Lysosomes Serve as the Cell’s Digestive System • The flow of membrane within the cell (F5.10 p. 87) Copyright © 2005 Pearson Prentice Hall, Inc. phagocytosis plasma membrane exocytosis lysosome fused with food vacuole food vacuole Golgi complex smooth endoplasmic reticulum rough endoplasmic reticulum nuclear envelope CELL STRUCTURE & FUNCTION – Membrane Synthesized in the Endoplasmic Reticulum Flows Through the Membrane System of the Cell • Vacuoles Serve Many Functions, Including Water Regulation, Support, and Storage – Freshwater Microorganisms Have Contractile Vacuoles • Contractile vacuoles (F5.11 p. 88) Copyright © 2005 Pearson Prentice Hall, Inc. contractile vacuole collecting ducts central reservoir pore CELL STRUCTURE & FUNCTION – Plant Cells Have Central Vacuoles • The central vacuole and turgor pressure in plant cells (F5.12 p. 89) Copyright © 2005 Pearson Prentice Hall, Inc. cytoplasm central vacuole cell wall plasma membrane CELL STRUCTURE & FUNCTION Mitochondria (F5.13 p. 90) Chloroplasts (F5.14 p. 91) Use Energy Stored in Capture Solar Energy Food Molecules Sites of Produce ATP Photosynthesis Copyright © 2005 Pearson Prentice Hall, Inc. outer membrane inner membrane intermembrane compartment matrix cristae 0.2 micrometer outer membrane inner membrane stroma thylakoid channel interconnecting thylakoids granum (stack of thylakoids) 1 micrometer CELL STRUCTURE & FUNCTION plastid starch globules 0.5 micrometer Plants Use Plastids for Storage (F5.15 p. 92) CELL STRUCTURE & FUNCTION plasma membrane microfilaments mitochondrion intermediate filaments ribosomes endoplasmic reticulum The Cytoskeleton: Provides Shape, Support, & Movement microtubule vesicle (F5.16 p. 92) microtubules (red) nucleus microfilaments (blue) microtubules (red) nucleus microfilaments (blue) central unfused pair of microtubules Protein "arms" CELL STRUCTURE & FUNCTION Paramecium x-section of cilium (9+2 arrangement) 0.1 micrometer fused pair of microtubules cilium plasma membrane basal body x-section of basal body (9+0 arrangement) Cilia and Flagella: Move the Cell Through Fluid or Move Fluid Past the Cell Cilia & flagella Structure (F5.17 p. 93) 0.1 micrometer CELL STRUCTURE & FUNCTION Cilium Propulsion of fluid power stroke Flagellum cilia lining trachea return stroke plasma membrane direction of locomotion propulsion of fluid flagellum of human sperm continuous propulsion Cilia and Flagella: Move the Cell Through Fluid or Move Fluid Past the Cell How cilia and flagella move (F5.18 p. 94)