Download Dr Asmat Salim MM 704 stem cells

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Transcript 
Core Course # MM 704
(Cellular and Molecular Pathology)
Cell cycle (Cell division), Cell-cell interaction, growth
and their control
Aging
Apoptosis
Tissue injury and repair mechanisms
Cell regenerating potential (Role of Stem Cells)
Mediators, modulators and messengers
Antioxidants in health and disease
Induced differentiation of stem cells by
small molecules
Strategies to enhance regenerative
potential of stem cells
Induced differentiation of stem cells by
small molecules

Direct Differentiation of ES Cells

Direct Differentiation of MSCs
Small Molecules and Cardiac Differentiation
Dexamethasone
Ascorbic acid
5-azacytidine
Zebularine
Retinoic acid
Nitric oxide
Difluoromethylornithine (DFMO)
Differentiation of the Adult Stem Cells into Cardiac
like cells by Small Molecules
Zebularine
Differentiation of the Adult Stem Cells into Cardiac
like cells by Small Molecules
Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x
Differentiation of the Adult Stem Cells into Cardiac
like cells by Small Molecules
Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x
Differentiation of the Adult Stem Cells into Cardiac like cells
by Small Molecules
Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x
Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x
Strategies to enhance regenerative potential
of stem cells
(a) Hypoxic Preconditioning
After Hypoxia
Before Hypoxia
After Reoxygenation
(b) Genetic Manipulation
e.g. Viral mediated
Engineered MSCs transdifferentiate into CMs
Circulation Res. Vol 99, 776-84. 2006
Transplantation of Modified Stem Cells
Myocardial infarction
Blocked blood vessel
Heart Attack
Dead muscle
Scar tissue
Cell Transplantation
Rat
Isolation of Mesenchymal
Stem Cells from rats
Infarction Model in Rats
Chemical or Genetic
Modification
Cell Transplantation
Histology
Functional Studies
Myogenic transdifferentiation of MSC at 4
weeks after cell transplantation
FITC actinin staining
Red PKH26 staining
DAPI Nuclear staining
Merge
Circulation Res. Vol 99, 776-784. 2006
References
Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755
5922.2012.00320.x
Circulation Res. Vol 99, 776-84. 2006
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