Download BIT495/595 Epigene cs Overview

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BIT495/595 Epigene/cs Overview: •  This course will integrate the use of standard assays with novel technologies to assess altera5ons in epigene5c markers during cell differen5a5on and stress responses. •  Emerging topics in epigene5c research will be discussed. •  Students will also gain experience working with mammalian 5ssue culture and a model plant system. Selected Lecture Topics: 1.  DNA methyla5on 2.  Histone modifica5ons and histone modifying enzymes 3.  Polycomb and Trithorax complexes 4.  Long-­‐range chroma5n interac5ons 5.  Noncoding RNA 6.  Manipula5ng epigene5cs Labs: 1. 
2. 
Mapping genomewide methyla5on paPerns in Medicago truncatula a.  Bisulfite-­‐sequencing: Library prepara5on
b.  Bisulfite-­‐sequencing: Data analysis
Monitoring changes in histone modifica5ons during differen5a5on by chroma5n immunoprecipita5on 3 weeks 1 week 3 weeks
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