Download Human Blood Typing Lab

Human Blood Typing
Lab
Objective:
1. Apply blood typing method to determine the
blood type for a sample of synthetic human
blood
2. Describe the appearance of agglutinated blood.
3. Explain how to determine a human blood type.
4. Using data, determine the blood type for each
person.
Human Blood Typing Vocabulary
blood type = blood group - Any of several immunologically distinct,
genetically determined classes of human blood which are based on the presence
or absence of certain erythrocyte surface antigens (glycoproteins) and are
clinically identified by characteristic agglutination reactions; for blood
transfusion purposes, the ABO and Rh blood group systems are the most
important.
agglutinin - A molecule, such as an antibody, which is capable of causing
agglutination (clumping) of a particular antigen, especially red blood cells or
bacteria.
agglutination - The clumping together of red blood cells, or bacteria, or other
particulate/cellular antigens, usually in response to a specific antibody.
The specific anti-serum (antibody) goes with a specific antigen
to cause agglutination. For example, B anti-serum and B antigen.
So if a red blood cell has the B antigen on its membrane, B anti-serum
Will cause agglutination. Click to see next slide.
“The Basis of Blood Typing”
Notice that the antibodies (Y-shaped structures)
attach to the red blood cells and then cause them to
stick together. If this is Type A, then the Y-shaped
structures are Anti-A serum (antibody A).
Blood contains antibodies in the liquid component,
plasma. The antibodies serve a protective function.
Why does Type AB blood have no antibodies.
Human ABO Blood Typing Explanation
Can you explain these
Photographs?
Results of Blood Typing Test After the Addition of Anti-serum
(antibody)
Sample
Anti-A
Anti-B
Anti-Rh
Blood
Type
Mr. Smith
Mr. Jones
Mr. Green
Ms.
Brown
Yes = agglutination
No = no agglutination
1.
2.
3.
4.
5.
6.
7.
Which sample(s) lacked the Rh antigen?
Which sample(s) lacks both A and B
antigens?
Which sample(s) contains only B
antibodies?
Which sample(s) contain no antibodies?
What is Mr. Brown’s blood type?
Who is the universal donor?
Could Mr. Jones give to Mr. Green?
What do the results say?
The antigens on the donor’s red blood
cell must not react with the
antibodies in the receiver’s body.
Safe Blood Donation: